biologia plantarum

Biologia plantarum 45:469-472, 2002 | DOI: 10.1023/A:1016246507339

Callus Induction and Plant Regeneration in Lemna Minor L.

B. Stefaniak¹, A. Woźny¹, I. Budna¹

¹ Department of General Botany, Institute of Experimental Biology, Adam Mickiewicz University, Poznań, Poland

Callus induction was obtained on Murashige and Skogg agar medium with 45 μM 2,4-dichlorophenoxyacetic acid under dark at 25°C. Among the four explant types investigated, the best callus induction was obtained from two-week old fronds to which a surgical incision was applied in the basal (meristematic) region. This treatment resulted in 89.11% of fronds producing callus which continued to proliferate for another 24 months. To obtain plant regeneration pieces of calluses were transferred onto Murashige and Skoog agar medium containing 22 μM indole-3-acetic acid and 4.6 μM kinetin and maintained under 16-h photoperiod (irradiance of 30 μmol m^-2 s^-1) at 23°C. Green fronds formed on all callus pieces. The regenerated fronds were later transferred onto Wang medium where they formed roots. The regenerated Lemna minor L. plants obtained through indirect organogenesis did not differ morphologically from individuals forming the stock collection.

Keywords: Lemnaceae; duckweed; frond; organogenesis

Subjects: auxins; cytokinins; 2,4-dichlorophenoxyacetic acid; duckweed, organogenesis; frond regeneration, duckweed; in vitro culture, bud and callus induction and growth; in vitro culture, clonal propagation; in vitro culture, nutrient media modification; in vitro culture, plant regeneration, organogenesis; Lemna minor

Published: September 1, 2002  Show citation