biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:489-493, 2002 | DOI: 10.1023/A:1022360230668

Propagation of Chelidonium majus L. by Somatic Embryogenesis

B. Vinterhalter1, D. Vinterhalter2
1 Plant Physiology Department, Institute for Biological Research "Siniša Stanković", Belgrade, Yugoslavia
2 Department of Biology, Faculty of Natural Sciences, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

Direct somatic embryogenesis in celandine (Chelidonium majus L.) was achieved in epicotyl explants of seedlings after prolonged cultivation on Murashige and Skoog medium with or without plant growth regulators. Somatic embryos developed into plantlets which entered additional cycles of somatic embryogenesis. Cultures consisting of plantlets with prolonged embryogenic potential were maintained for five years on plant growth regulator free medium. Embryos which developed into rooted plantlets could be acclimated in a glasshouse enabling thus a continuous propagation scheme to be established.

Keywords: celandine; growth regulator-free medium; medicinal plant
Subjects: celandine, somatic embryogenesis; Chelidonium majus; in vitro culture, growth regulator-free medium; in vitro culture, nutrient media modification; in vitro culture, somatic embryogenesis; somatic embryogenesis

Published: December 1, 2002  Show citation

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Vinterhalter, B., & Vinterhalter, D. (2002). Propagation of Chelidonium majus L. by Somatic Embryogenesis. Biologia plantarum, 45(4), 489-493. doi: 10.1023/A:1022360230668
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