biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:495-502, 2002 | DOI: 10.1023/A:1022323221513

Effect of Callus Induction Media on Morphology of Embryogenic Calli in Rice Genotypes

K.B.R.S. Visarada1, M. Sailaja2, N.P. Sarma2
1 National Research Centre for Sorghum, Hyderabad, India
2 Directorate of Rice Research, Rajendranagar, Hyderabad, India

Effects of four culture media on callus induction, regeneration and number of plants per unit culture were studied with mature seeds from five indica rice genotypes as explants. Based on the morphology, the calli were classified into four types as I to IV. Type I and type II are most suited to initiate suspension cultures or as target material for transformation. Number of plants regenerated per unit culture, formation of easily dissociating cell clusters and frequency of type I and type II calli were highest on NBKNB medium. Thus NBKNB medium is suitable for in vitro culture of even the hitherto recalcitrant indica genotypes.

Keywords: medium composition; Oryza sativa; regeneration
Subjects: in vitro culture, bud and callus induction and growth; in vitro culture, nutrient media modification; Oryza sativa; rice, genotypes, embryogenic calli

Published: December 1, 2002  Show citation

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Visarada, K.B.R.S., Sailaja, M., & Sarma, N.P. (2002). Effect of Callus Induction Media on Morphology of Embryogenic Calli in Rice Genotypes. Biologia plantarum, 45(4), 495-502. doi: 10.1023/A:1022323221513
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    References

    1. Abe, T., Futsuhara, Y.: Varietal differences in plant regeneration from root callus tissues of rice.-Jap. J. Breed. 34: 147-155, 1984. Go to original source...
    2. Abe, T., Futsuhara, Y.: Genotypic variability to callus formation and plant regeneration in rice (Oryza sativa L).-Theor. appl. Genet. 72: 3-10, 1986. Go to original source...
    3. Bec, S., Chen, L., Ferriere, M.N., Lagavre, T., Fauquet, C., Guiderdoni, E.: Comparative histology of microprojectile mediated gene transfer of embryogenic calli in japonica rice (Oryza sativa) L.: influence of the structural organisation of target tissues on genotype transformation ability.-Plant Sci. 138: 177-190, 1998. Go to original source...
    4. Bhaskaran, S., Smith, R.H.: Regeneration in cereal tissue culture: a review.-Crop Sci. 30: 1328-1337, 1990. Go to original source...
    5. Chowdhry, C.N., Tyagi, A.K., Maheswari, N., Maheswari, S.C.: Effect of L-proline and L-tryptophan on somatic embryogenesis and plantlet regeneration of rice (Oryza sativa L. cv. Pusa 169).-Plant Cell Tissue Organ Cult. 32: 357-361, 1993. Go to original source...
    6. Dinghou, L., Komamine, A.: Some regulations of somatic embryogenesis and organogenesis in indica rice.-J. trop. subtrop. Bot. 2: 70-78, 1994.
    7. Grimes, H.D., Hodges, T.K.: The inorganic NO3 -:NH4 + ratio influences plant regeneration and auxin sensitivity in primary callus derived from immature embryos of indica rice (Oryza sativa L.).-J. Plant Physiol. 136: 362-367, 1990. Go to original source...
    8. Heyser, J.W., Dykes, T.A., DeMott, K.J., Nabors. M.W.: High frequency long term regeneration of rice callus culture.-Plant Sci. Lett. 29: 175-182, 1983. Go to original source...
    9. Jain, R.K., Jain, S., Wang, B., Wu, R.: Optimisation of biolistic method for transient gene expression and production of agronomically useful Basmati rice plants.-Plant Cell Rep. 15: 963-968, 1996. Go to original source...
    10. Jones, T.J., Rost, T.L.: The developmental anatomy and ultrastructure of somatic embryos from rice Oryza sativa (L.) scutellum epithelial cells.-Bot. Gaz. 150: 41-49, 1989. Go to original source...
    11. Khanna, H., Raina, S.K.: Enhanced in vitro plantlet generation from mature embryo-derived primary callus of a Basmati rice cultivar through modification of nitrate-nitrogen and ammonium-nitrogen concentrations.-J. Plant Biochem. Biotechnol. 6: 85-89, 1997. Go to original source...
    12. Koetje, D.S., Grimes, H.D., Wang, Y.C., Hodges, T.K.: Regeneration of indica rice (Oryza sativa L.) from primary callus derived from immature embryos.-J. Plant Physiol. 135: 184-190, 1989. Go to original source...
    13. Krishnaraj, S., Vasil, I.K.: Somatic embryogenesis in herbaceous monocots.-In: Thorpe, T.A. (ed.): In Vitro Embryogenesis in Plants. Pp. 417-470. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...
    14. Lai, K.L., Liu, L.F.: Further studies on the variability of plant regeneration from young embryo callus cultures of rice plants (Oryza sativa L.).-Jap. J. Crop Sci. 55: 41-46, 1986. Go to original source...
    15. Leifert, C., Murphy, K.D., Lumsden, P.J.: Mineral and carbohydrate nutrition of plant cell and tissue cultures.-Crit. Rev. Plant Sci. 14: 83-109, 1995. Go to original source...
    16. Maeda, E., Chen, M.H., Inoue, M.: Rice: regeneration of plants from callus cultures.-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Vol. 2. Pp. 106-122. Springer-Verlag, Berlin-Heidelberg 1986. Go to original source...
    17. Maggioni, L., Lusardi, M.C., Lupotto, E.: Effects of cultural conditions on callus induction and plant regeneration from mature and immature embryos of rice varieties (Oryza sativa L.).-J. Genet. Breed. 43: 99-106, 1989.
    18. Moura, D.S., Zapata-Arias, F.J., Ando, A., Tulmann, A.: Plant regeneration from protoplasts isolated from primary calli using mature embryos of two Brazilian rice cultivars.-Euphytica 94: 1-5, 1997. Go to original source...
    19. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    20. Narciso, J.O., Hattori, K.: Histological observation of callus morphology in rice.-Int. Rice Res. Notes 21: 36, 1996.
    21. Poonsapaya, P., Nabors, M.W., Wright, K., Vajrabhaya, M.: A comparison of methods for callus culture and plant regeneration of RD25 rice in two laboratories.-Plant Cell Tissue Organ Cult. 16: 175-186, 1989. Go to original source...
    22. Raval, M., Chattoo, B.B.: Role of media constituents and proline in callus growth somatic embryogenesis and regeneration of Oryza sativa cv. indica.-Indian J. exp. Biol. 31: 600-603, 1993.
    23. Rueb, S., Leneman, M., Schilperoort, R.A., Hensgenes, L.A.M.: Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.).-Plant Cell Tissue Organ Cult. 36: 259-264, 1994. Go to original source...
    24. Sivamani, E., Shen, P., Opalka, N., Beachy, R.N., Fauquet, C.M.: Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using biolistic method.-Plant Cell Rep. 15: 322-327, 1996. Go to original source...
    25. Vain, P., Yean, H., Flament, P.: Enhancement of production and regeneration of embryogenic type II callus in Zea mays L. by AgNO3.-Plant Cell Tissue Organ Cult. 18: 143-151, 1989. Go to original source...
    26. Vasil, V., Vasil, I.K.: Isolation and maintenance of embryogenic callus cultures of Gramineae.-In: Vasil, V., Vasil, I.K.: Cell Culture and Somatic Cell Genetics of Plants. Vol. 1. Pp. 152-158, Academic Press, Orlando 1984. Go to original source...
    27. Visarada, K.B.R.S., Vijaya Kumar, C.H.M., Sarma, N.P.: Analysis of variation in tissue culture response for identification of indica rice genotypes suitable for transformation.-J. Genet. Breed. 55: 125-134, 2001.
    28. Yoshida, S., Forno, D.A., Cock, J.H., Gomez, K.A.: Laboratory Manual for Physiological Studies in Rice.-International Rice Research Institute, Los Baños 1976.

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