biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:517-522, 2002 | DOI: 10.1023/A:1022312806492

Malate Dehydrogenase, Alcohol Dehydrogenase, and 6-Phosphogluconate Dehydrogenase Isozymes of Zea mays L. × Tripsacum dactyloides L. Hybrids and Parents

V. Tsanev1, R. Vladova1, K. Petkolicheva1, B. Kraptchev1, C. Milanov1
1 Acad. D. Kostoff Institute of Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria

Electrophoretic patterns of malate dehydrogenase (Mdh), alcohol dehydrogenase (Adh), and 6-phosphogluconate dehydrogenase (Pgd) of Zea mays L. × Tripsacum dactyloides L. hybrids and their parents were compared. The components of enzymes specific to T. dactyloides may be used as markers to identify the following T. dactyloides chromosomes in the hybrids: Tr 16 (Mdh 2 and Pdg 1), Tr 7, and/or Tr 13 (Adh 2). The isozymes of Mdh 2 are supposed as a possible biochemical marker to evaluate the introgression of genes, determining an apomictic mode of reproduction from T. dactyloides (localized on Tripsacum 16 chromosome) into Z. mays. The isozymes may be used as markers for the identification of maize chromosomes 1 and 6 in the hybrids as well. Chromosome count taken on the examined hybrids showed the addition of 9 to 13 chromosomes of T. dactyloides to maize chromosome complement.

Keywords: biochemical markers; chromosome complement; electrophoresis; isozymes; maize
Subjects: alcohol dehydrogenase; chromosome; isozymes as markers; maize, enzymes, hybrids; malate dehydrogenase; markers; 6-phosphogluconate dehydrogenase; Tripsacum dactyloides; Zea x Tripsacum hybrids and parents, enzymes; Zea mays

Published: December 1, 2002  Show citation

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Tsanev, V., Vladova, R., Petkolicheva, K., Kraptchev, B., & Milanov, C. (2002). Malate Dehydrogenase, Alcohol Dehydrogenase, and 6-Phosphogluconate Dehydrogenase Isozymes of Zea mays L. × Tripsacum dactyloides L. Hybrids and Parents. Biologia plantarum, 45(4), 517-522. doi: 10.1023/A:1022312806492
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