biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:557-562, 2002 | DOI: 10.1023/A:1022377126056

Response of Tea Plants to Water Stress

U. Chakraborty1, S. Dutta1, B.N. Chakraborty2
1 Plant Biochemistry Laboratory, Department of Botany, University of North Bengal, Siliguri, India
2 Immuno-Phytopathology Laboratory, Department of Botany, University of North Bengal, Siliguri, India

Two-year-old potted plants of six Camellia sinensis cultivars (TV-18, TV-26, UPASI-3, UPASI-26, T-78 and HV-39) were subjected to water stress for 4, 8 and 12 d. Relative water content (RWC) of leaves of all cultivars declined with water stress, but in the two drought tolerant cultivars (UPASI-3 and UPASI-26), higher RWC were maintained in comparison to the others. Phenol content and activities of phenylalanineammonialyase, polyphenoloxidase and peroxidase initially increased, but decreased during extended drought. Chlorophyll contents decreased, whereas proline contents increased during water stress. SDS-PAGE analysis of proteins revealed increased accumulation of proteins of intermediate molecular masses (42 - 44 kDa) and low molecular masses (14 - 26 kDa). After 12 d of water stress, most of these proteins disappeared in T-78 and HV-39, but in the other cultivars they were still detectable.

Keywords: Camellia sinensis; chlorophyll; peroxidase isozymes; phenols; phenylalanineammonialyase; polyphenoloxidase; proline; protein; relative water content
Subjects: Camellia sinensis; drought stress, tolerance, resistance; peroxidase; phenylalanineammonialyase; polyphenoloxidase; proline; relative water content; tea, water stress, enzyme isozymes; water stress, chlorophyll

Published: December 1, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Chakraborty, U., Dutta, S., & Chakraborty, B.N. (2002). Response of Tea Plants to Water Stress. Biologia plantarum, 45(4), 557-562. doi: 10.1023/A:1022377126056
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Andrade, J.L., Larque-Saavedra, A., Truju, C.L.: Proline accumulation in four cultivars of Phaseolus vulgaris L. with different drought resistance.-Phyton 57: 149-157, 1995.
    2. Barathi, P., Sundar, D., Reddy, R.A.: Changes in mulberry leaf metabolism in response to water stress.-Biol. Plant. 44: 83-87, 2001. Go to original source...
    3. Bates, H.S., Waldren, R.P., Treare, I.D.: Rapid estimation of free proline for water stress determination.-Plant Soil 39: 205-207, 1973. Go to original source...
    4. Bradford, K.: A rapid and sensitive method for the quantification of microgramme quantities of protein utilising the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    5. Carceller, M., Prystupa, P., Lemcoff, J.H.: Remobilization of proline and other nitrogen compounds from senescing leaves of maize under water stress.-J. Agron. Crop Sci. 183: 61-66, 1999. Go to original source...
    6. Chakraborty, B.N., Basu, P., Das, R., Saha, A., Chakraborty, U.: Detection of cross reactive antigens between Pestalotiopsis theae and tea leaves and their cellular location.-Ann. appl. Biol. 127: 11-21, 1995. Go to original source...
    7. Chakraborty, U., Chakraborty, B.N., Kapoor, M.: Changes in the levels of peroxidase and phenyl alanine ammonia lyase in Brassica napus cultivars showing variable resistance to Leptosphaeria maculans.-Folia microbiol. 38: 491-496, 1993. Go to original source...
    8. Chen, C., Belanger, R.R., Benhamou, M., Paulitz, T.: Defense enzyme induced in cucumber roots by treatment with plant growth promoting rhizobacteria (PGPR) and Pythium aphanidermatum.-Physiol. mol. Plant Pathol. 56: 13-23, 2000. Go to original source...
    9. Curtis, M.D., Rac, A.L., Rasu, A.G., Harrison, H.J., Manners, J.M.: A peroxidase gene promoter induced by phytopathogens and methyl jasmonate in transgenic plants.-Mol. Plant Microbiol. Interact. 10: 326-338, 1997. Go to original source...
    10. Davis, B.J.: Disc electrophoresis methods and application to human serum proteins.-Ann. nat. Acad. Sci. 121: 404, 1967. Go to original source...
    11. Farooqui, A.H.A., Kumar, R., Fatima, S., Sharma, S.: Response of different genotype of lemon grass (Cymbopogon flexuosus and C. pendulus) to water stress.-J. Plant Biol. 27: 277-282, 2000.
    12. Gardner, W.H.: Water contents.-In: Blank, C.A. (ed.): Methods of Soil Analysis. Part I. Pp. 84. American Society for Agronomy, Madison 1965.
    13. Girousse, C., Bournoville, R., Bonnenain, N.: Water deficit induced changes in concentrations in proline and some other amino acids in the phloem sap of alfalfa.-Plant Physiol. 111: 109-113, 1996. Go to original source...
    14. Harborne, J.B.: Phytochemical Methods.-Chapman and Hall, London 1973.
    15. Janave, M.T.: Enzymatic degradation of chlorophyll in cavendish bananas - in vitro evidence for two independent degradative pathways.-Plant Physiol. Biochem. 35: 837-846, 1997.
    16. Kaur, M., Deshmukh, K.B.: Photosynthetic activities of cowpea plants infected with Erysiphe polygoni.-Indian Phytopathol. 33: 344-345, 1980.
    17. Kramer, P.J., Boyer, J.S.: Water Relations of Plants and Soils.-Academic Press, San Diego 1995. Go to original source...
    18. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    19. Leinhos, V., Bergmann, H.: Changes in the yield, lignin content and protein patterns of barley (Hordeum vulgare cv. Alexis) induced by drought stress.-Angew. Bot. 69: 206-210, 1995.
    20. Liyanage, A.C., Penyasiri, P.A.N.: High performance liquid chromatography (HPLC) of chlorophylls in tea (Camellia sinensis).-Sri Lanka J. Tea Sci. 62: 32-37, 1993.
    21. Mahadevan, A., Sridhar, R.: Methods in Physiological Plant Pathology. 2nd Edition.-Sivakami Publications, Madras, 1982.
    22. Nanjo, T., Kobayashi, M., Yoshiba, Y., Sanada, Y., Wada, K., Tsukaya, H., Kakubari, Y., Yamaguchi-Shinozaki, K., Shinozaki, K.: Biological functions of proline in morphogenesis and osmotolerance revealed in antisense Arabidopsis thaliana.-Plant J. 18: 185-193, 1999. Go to original source...
    23. Ng'etich, W.K.: Responses of tea to environment.-Tea 18: 149-155, 1997.
    24. Pareek, A., Singla, S.L., Grover, A. Analysis of stress proteins at four different developmental stages in field-grown rice, Oryza sativa L. (cv. Pusa 169) plants.-Curr. Sci. 76: 81-86, 1999.
    25. Sanchez, F.J., Manzanares, M., Deandres, E.F., Tenorio, J.L., Ayerbe, L.: Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress.-Field Crops Res. 59: 225-235, 1998. Go to original source...
    26. Schonfield, M.A., Johnson, R.C., Carver, B.F., Mornhinweg, W.: Water relations in winter wheat as drought resistance indicators.-Crop Sci. 28: 526-531, 1988. Go to original source...
    27. Sinha, K.M., Sachdev, A., Johri, R.P., Mehta, S.L. Stress induced polypeptides in Lathyrus sativus.-J. Plant Biochem. Biotechnol. 8: 47-51, 1999. Go to original source...

    Return to the content