biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:569-573, 2002 | DOI: 10.1023/A:1022381226965

Light-Dependent Ammonium Ion Toxicity of Rice Leaves in Response to Phosphinothricin Treatment

Y.-C. Tsai1, C.H. Kao1
1 Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

Ammonium ion accumulation in detached rice leaves treated with phosphinothricin (PPT), an inhibitior of glutamine synthetase (GS), was investigated in the light and darkness. PPT treatment increased NH4+ content and induced toxicity in rice leaves in the light but not in darkness, suggesting the importance of light in PPT-induced NH4+ toxicity in detached rice leaves. PPT treatment in the light resulted in a decrease of activities of the cytosolic form of GS and the chloroplastic form of GS. The photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea reduced NH4+ accumulation induced by PPT in the light. In darkness, PPT-induced NH4+ accumulation and toxicity were observed in the presence of glucose or sucrose.

Keywords: glutamine synthetase; Oryza sativa
Subjects: ammonium ion toxicity, light dependence; glutamine synthetase; Oryza sativa; phosphinothricin; rice, ammonium ion toxicity

Published: December 1, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Tsai, Y.-C., & Kao, C.H. (2002). Light-Dependent Ammonium Ion Toxicity of Rice Leaves in Response to Phosphinothricin Treatment. Biologia plantarum, 45(4), 569-573. doi: 10.1023/A:1022381226965
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    2. Givan, C.V.: Metabolic detoxification of ammonia in tissues of higher plants.-Phytochemistry 18: 375-382, 1979. Go to original source...
    3. Hirel, B., Gadal, P.: Glutamine synthetase in rice. A comparative study of the enzyme from root and leaves.-Plant Physiol. 66: 619-623, 1980. Go to original source...
    4. Hurst, P.L., King, G.A., Borst, W.M.: Postharvest inhibition of glutamine synthetase activity with phosphinothricin reduces the shelf-life of asparagus.-Postharvest Biol. Technol. 3: 327-334, 1993. Go to original source...
    5. Johnson, C.M., Stout, P.R., Broyer, T.C., Carlton, A.B.: Comparative choline requirements of different plant species.-Plant Soil 8: 337-353, 1957. Go to original source...
    6. Lancien, M., Gadal, P., Hodges, M.: Enzyme redundancy and the importance of 2-oxoglutarate in higher plant ammoniun assimilation.-Plant Physiol. 123: 817-824, 2000. Go to original source...
    7. Lea, P.J., Ridley, S.M.: Glutamine synthetase and its inhibition.-In: Dodge, A.D. (ed.): Herbicide and Plant Metabolism. Pp. 137-170. Cambridge University Press, New York 1990. Go to original source...
    8. Lea, P.J., Joy, K.W., Ramos, J.P., Guerrero, M.G.: The action of 2-amino-4-(methylphosphinyl)-butanoic acid (phosphinothricin) and its 2-oxo-derivative on the metabolism of cyanobacteria and higher plants.-Phytochemistry 23: 1-6, 1980. Go to original source...
    9. Lin, C.C., Kao, C.H.: Disturbed ammonium assimilation is associated with growth inhibition of roots in rice seedlings caused by NaCl.-Plant Growth Regul. 18: 233-238, 1996. Go to original source...
    10. Lin, J.-N., Wang, J.-W., Kao, C.H.: Effect of abscisic acid and water stress on the senescence of detached rice leaves.-Biol. Plant. 42: 313-316, 1999. Go to original source...
    11. Lutts, S., Majerus, V., Kinet, J.-M.: NaCl effects on proline metabolism in rice (Oryza sativa) seedling.-Plant Physiol. 105: 450-458, 1999. Go to original source...
    12. Lydon, J., Duke, S.O.: Inhibitors of glutamine biosynthesis.-In: Singh, B.K. (ed.): Plant Amino Acids, Pp. 445-464. Marcel Dekker, New York 1999.
    13. Miflin, B.J., Lea, P.J.: The pathway of nitrogen assimilation in plants.-Phytochemistry 15: 873-885, 1976. Go to original source...
    14. Oaks, A., Stulen, I., Jones, K., Winspear, M.J., Boesel, I.L.: Enzymes of nitrogen assimilation in maize roots.-Planta 148: 477-484, 1980. Go to original source...
    15. Perez-Garcia, A., de Vicente, A., Canton, F.R., Cazorla, F.M., Codina, J.C., Garcia-Gutierrez, A., Canovas, F.M.: Light-dependent changes of tomato glutamine synthetase in response to Pseudomonas syringae infestion or phosphinothricin treatment.-Physiol. Plant. 102: 377-384, 1998. Go to original source...
    16. Tachibana, K., Watanabe, T., Seikizawa, Y., Takematsu, T.: Accumulation of ammonia in plants treated with bialaphos.-J. Pestic. Sci. 11: 33-37, 1986. Go to original source...
    17. Wild, A., Sauer, H., Ruhle, W.: The effect of phosphinothricin (glufosinate) on photosynthesis. I. Inhibition of photosynthesis and accumulation of ammonia.-Z. Naturforsch. 42c: 263-269, 1987. Go to original source...
    18. Wintermans, J.F.G.M., De Mots, A.: Spectrophotometric characteristics of chlorophyll a and b and their pheophytins in ethanol.-Biochim. biophys. Acta 109: 448-453, 1965. Go to original source...

    Return to the content