biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:575-580, 2002 | DOI: 10.1023/A:1022333311035

Glucose-6-Phosphate Dehydrogenase/6-Phosphogluconate Dehydrogenase Ratio and the Glucose-6-Phosphate, 6-Phosphogluconate and Fructose-6-Phosphate Contents in Tobacco Plants Infected with Potato Virus Y

L. Šindelář1, M. Šindelářová1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic

The ratio of activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (G6P DH/6PG DH), and the contents of glucose-6-phosphate (G6P), 6-phosphogluconate (6PG) and fructose-6-phosphate (F6P) were studied at various stages of potato virus Y (PVY) multiplication in Nicotiana tabacum cv. Samsun. G6P DH/6PG DH increased through the experiment from 0.42 to 0.53 in leaves of healthy tobacco, and up to 0.59 in PVY systemically infected leaves. However, these ratios in the ruptured protoplast preparations, and the chloroplast and cytosol fractions of healthy protoplasts were similar to that from infected ones. The ratio lower than 1, found in the healthy and/or PVY- infected leaf tissues and in the infected protoplasts as well, confirms the assumption that G6P DH is the control enzyme of oxidative pentosephosphate pathway not only in the healthy but also in the infected plants. The contents of G6P, 6PG and F6P in the period of the highest PVY multiplication were strongly decreased (to 30 - 50 % when compared with control healthy leaves) and were negatively correlated with the G6P DH and 6PG DH activities.

Keywords: protoplast; chloroplast; cytosol; regulation; oxidative pentosephosphate pathway; Nicotiana tabacum
Subjects: glucose-6-phosphate dehydrogenase; Nicotiana tabacum; pentosephosphate pathway, oxidative; 6-phosphogluconate dehydrogenase; potato virus Y, glucose-6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase ratio; tobacco, G6P dehydrogenase/6-phosphogluconate dehydrogenase ratio, potato virus Y

Published: December 1, 2002  Show citation

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Šindelář, L., & Šindelářová, M. (2002). Glucose-6-Phosphate Dehydrogenase/6-Phosphogluconate Dehydrogenase Ratio and the Glucose-6-Phosphate, 6-Phosphogluconate and Fructose-6-Phosphate Contents in Tobacco Plants Infected with Potato Virus Y. Biologia plantarum, 45(4), 575-580. doi: 10.1023/A:1022333311035
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