Biologia plantarum 45:585-588, 2002 | DOI: 10.1023/A:1022386816748
Two-Dimensional Electrophoretic Analysis of Salicylic Acid-Induced Changes in Polypeptide Pattern of Barley Leaves
- 1 "M. Popov" Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
The salicylic acid-induced changes in the polypeptide patterns of barley (Hordeum vulgare L.) leaves have been analysed using two-dimensional gel electrophoresis. An optimized 2-D PAGE protocol was used and gave reproducible 2-D gels from leaf crude protein extracts with a high number of detected polypeptides. When applied for 24 h SA affected the expression of a number of soluble proteins. Most of them appeared to be down-regulated. Although no abundant expression of specific proteins was observed, we detected three polypeptides that were present only in SA-treated leaves.
Keywords: 2-D PAGE; Hordeum vulgare; leaf proteins
Subjects: barley, polypeptide pattern, salicylic acid; Hordeum vulgare; salicylic acid, polypeptide pattern induction
Published: December 1, 2002 Show citation
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Metodiev, M.V., Kicheva, M.I., Stoinova, Z.G., & Popova, L.P. (2002). Two-Dimensional Electrophoretic Analysis of Salicylic Acid-Induced Changes in Polypeptide Pattern of Barley Leaves. Biologia plantarum, 45(4), 585-588. doi: 10.1023/A:1022386816748
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