biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 45:597-599, 2002 | DOI: 10.1023/A:1022343101727

Effect of NaCl and Proline on Bean Seedlings Cultured in vitro

Y. Demir1, I. Kocaçalişkan2
1 Atatürk Üniversitesi, Erzurum, Turkey
2 Dumlupinar Üniversitesi, Kütahya, Turkey

Effects of NaCl (150 mM), proline (10 mM) and their combination on growth and contents of chlorophyll, proline and protein of bean (Phaseolus vulgaris cv. Kizilhaç) seedlings in vitro were investigated. NaCl decreased seedling growth. Proline added to control seedlings did not change seedling growth but decreased chlorophyll and increased protein contents. When proline added to NaCl-treated seedlings growth was increased in comparison with NaCl-treated only. Thus, proline alleviated salinity stress in bean seedlings.

Keywords: embryo culture; Phaseolus vulgaris; salt stress
Subjects: in vitro culture, somatic embryogenesis; Phaseolus vulgaris; proline; salt stress, proline; somatic embryogenesis

Published: December 1, 2002  Show citation

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Demir, Y., & Kocaçalişkan, I. (2002). Effect of NaCl and Proline on Bean Seedlings Cultured in vitro. Biologia plantarum45(4), 597-599. doi: 10.1023/A:1022343101727
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References

  1. Ahmad, I., Larher, F., Mann, A.F., McNally, S.F., Stewart, G.R.: Nitrogen metabolism of halophytes. IV. Characteristics of glutamine synthetase from Triglochin maritima.-New Phytol. 91: 585-595, 1982. Go to original source...
  2. Arnon, D.L.: Copper enzymes in isolated chloroplasts PPO in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
  3. Bates, L.S.; Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies.-Plant Soil 39: 205-207, 1973. Go to original source...
  4. Bernard, R., Oaks, A.: Metabolism of proline in maize root tips.-Can. J. Bot. 48: 1155-1158, 1969. Go to original source...
  5. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantites of protein utulizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
  6. Fukutoku, Y., Yamada, Y.: Sources of prolin-nitrogen in water stressed soybean (Glycine max). II. Fate of 15N-labelled protein.-Physiol. Plant. 61: 622-628, 1984. Go to original source...
  7. Hanson, A.D., Hitz, W.D.: Metabolic responses of mesophytes to plant water deficits.-Annu. Rev. Plant Physiol. 33: 163-203, 1982. Go to original source...
  8. Hurkman, W.J., Tanaka, C.K., Dupont, F.M.: The effects of salt stress on polypeptides in membrane fractions from barley roots.-Plant Physiol. 88: 1263-1273, 1988. Go to original source...
  9. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
  10. Pandey, R., Ganapathy, P.S.: The proline enigma: NaCl-tolerant and NaCl-sensitive callus lines of Cicer arietinum.-Plant Sci. 401: 13-17, 1985. Go to original source...
  11. Schobert, B., Tschesche, H.: Unusual solution properties of proline and its interaction with proteins.-Biochem. biophys. Acta 541: 270-277, 1978. Go to original source...
  12. Stewart, G.R., Lee, J.A.: The role of proline accumulation in halophytes.-Planta 120: 279-289, 1974. Go to original source...
  13. Tipirdamaz, R., Çakirlar, H.: Effects of salt and water stress on proportionally water content, proline and betain in two wheat (Triticum aestivum L.) varieties grown in Turkey.-Doğa-Tr. J. Biol. 14: 125-148, 1990.
  14. Tipirdamaz, R., Karakullukçu, Ş.: Effects of proline and glycinebetaine on growth and some internal solute changes of cultured tomato embryos in salt conditions.-Doğa-Tr. J. Bot. 17: 57-64, 1993.
  15. Wyn Jones, R.G., Storey, R.: Salt stress and comparative physiology in the Gramineae. IV. Comparison of salt stress in Saptina tawnsendri and three barley cultivars.-Aust. J. Plant Physiol. 5: 839-850, 1978. Go to original source...