biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:1-5, 2003 | DOI: 10.1023/A:1027360326886

Restart of Lignification in Micropropagated Walnut Shoots Coincides with Rooting Induction

B. Bisbis1, C. Kevers1, M. Crevecoeur2, J. Dommes1, T. Gaspar1
1 Biologie Moléculaire et Hormonologie Végétales, Université de Liège, Institut de Biologie Végétale, Sart Tilman, Liège, Belgium
2 Physiologie et Biochimie Végétales, Université de Genève, Genève 4, Switzerland

The lignin content of walnut shoots did not change during in vitro shoot multiplication. Lignin content started to increase as soon as shoots were passed to a rooting medium with auxin. Exogenous auxin (applied for rooting) caused a transient elevation of the endogenous free indoleacetic acid (IAA) content with a simultaneous decrease of peroxidase activity. These events typically marked the completion of the rooting inductive phase (before any visible histological event, that is before the cell divisions beginning the rooting initiation phase). This meant that either the given exogenous auxin or the endogenous IAA has served as signal for the stimulation of lignification. Continued increase of lignification in the shoots required completion of root formation; this increase indeed was slown down when root emergence did not occur. It was further shown that lignification varied conversely to the content of the soluble phenol content, itself apparently being related to the activity of phenylalanine ammonia-lyase activity.

Keywords: auxin; Juglans regia × J. nigra; in vitro culture; peroxidase
Subjects: auxins; in vitro culture, micropropagation; Juglans regia × Juglans nigra; lignification, micropropagated shoots; phenylalanine ammonia-lyase; rooting in vitro; walnut, micropropagation

Published: July 1, 2003  Show citation

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Bisbis, B., Kevers, C., Crevecoeur, M., Dommes, J., & Gaspar, T. (2003). Restart of Lignification in Micropropagated Walnut Shoots Coincides with Rooting Induction. Biologia plantarum, 46(1), 1-5. doi: 10.1023/A:1027360326886
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    References

    1. Aloni, R.: The induction of vascular tissues by auxin and cytokinin.-In: Davies, J.P. (ed.): Plant Hormones. Physiology, Biochemistry and Molecular Biology. Pp. 531-546. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...
    2. Bolwell, G.P.: Molecular aspects of the biosynthesis of extracellular matrix during xylogenesis and signaling.-In: Greppin, H., Penel, C., Simon, P. (ed.): Travelling Shot on Plant Development. Pp. 255-273. University of Geneva, Geneva 1997.
    3. Caboni, E., Tonelli, M.G., Lauri, P., Iacouacci, P., Kevers, C., Damiano, C., Gaspar, Th.: Biochemical aspects of almond microcuttings related to in vitro rooting ability.-Biol. Plant. 39: 91-97, 1997. Go to original source...
    4. Davies, W.J., Jeffcoat, B.: Importance of Root to Shoot Communication in the Responses to Environmental Stress.-British Soc. Plant Growth Regul., Bristol 1990.
    5. Degroote, D.K., Larson, P.R.: Correlation between net auxin and secondary xylem development in young Populus deltoides.-Physiol. Plant. 60: 459-466, 1984. Go to original source...
    6. Denne, M.P., Wilson, J.E.: Some quantitative effects of indoleacetic acid on the wood production and tracheid dimension of Picea.-Planta 134: 223-228, 1977. Go to original source...
    7. Driver, J.A., Kuniyuki, A.H.: In vitro propagation of Paradox walnut rootstock.-HortScience 19: 507-509, 1984. Go to original source...
    8. Druart, Ph., Kevers, C., Boxus, Ph., Gaspar, Th.: In vitro promotion of root formation by apple shoots through darkness effect on endogenous phenols and peroxidases.-Z. Pflanzenphysiol. 108: 429-436, 1982. Go to original source...
    9. Falasca, G., Reverberi, M., Lauri, P., Caboni, E., De Stradis, A., Altamura, M.M.: How Agrobacterium rhizogenes triggers de novo root formation in a recalcitrant woody plant: an integrated histological, ultrastructural and molecular analysis.-New Phytol. 145: 77-93, 2000. Go to original source...
    10. Ferrer, M.A., Pedreno, M.A., Ros Barcelò, A., Muñoz, R.: The cell wall localization of two strongly basic isoperoxidases in etiolated Lupinus albus hypocotyls and its significance in coniferyl alcohol oxidation and indole-3-acetic acid catabolism.-J. Plant Physiol. 139: 611-616, 1992. Go to original source...
    11. Gaspar, Th., Hausman, J.F., Faivre-Rampant, O., Kevers, C., Dommes, J.: Auxins in the biology of roots.-In: Waisel, Y., Eshel, A., Kafkafi, U. (ed.): Plant Roots. The Hidden Half. 3rd Ed. Pp. 383-403. Marcel Dekker, New York 2002. Go to original source...
    12. Gaspar, Th., Kevers, C., Hausman, J.F.: Indissociable chief factors in the inductive phase of adventitious rooting.-In: Altman, A., Waisel, Y. (ed.): Biology of Root Formation and Development. Pp. 55-63. Plenum Press, New York 1997. Go to original source...
    13. Gaspar, Th., Kevers, C., Hausman, J.F., Ripetti, V.: Peroxidase activity and endogenous free auxin during adventitious root formation.-In: Lumsden, P.J., Nicholas, J.R., Davis, W.J. (ed.): Physiology, Growth and Development of Plants in Culture. Pp. 289-298. Kluwer Academic Publishers, Dordrecht 1994. Go to original source...
    14. Gatineau, F., Fouché, J.G., Kevers, C., Hausman, J.F., Gaspar, Th.: Quantitative variations of indolyl compounds including IAA, IAA-aspartate and serotonin in walnut microcuttings during root induction.-Biol. Plant. 39: 131-137, 1997. Go to original source...
    15. Hausman, J.F., Evers, D., Kevers, C., Gaspar, Th.: Internal controls of root induction in poplar shoots raised in vitro.-J. appl. Bot. 71: 104-107, 1997.
    16. Heloir, M.C., Kevers, C., Hausman, J.F., Gaspar, Th.: Changes in the concentrations of auxins and polyamines during rooting of in vitro-propagated walnut shoots.-Tree Physiol. 16: 515-519, 1996. Go to original source...
    17. Jay-Allemand, C., Capelli, P., Cornu, D.: Root development of in vitro hybrid walnut microcuttings in a vermiculite-containing geltrite medium.-Sci. Hort. 51: 335-342, 1992. Go to original source...
    18. Kevers, C., Bringaud, C., Hausman, J.F., Gaspar, Th.: Putrescine involvement in the inductive phase of walnut shoots rooting in vitro.-Saussurea 28: 47-57, 1997a.
    19. Kevers, C., Gaspar, Th.: Soluble, membrane and wall peroxidases, phenylalanine ammonia-lyase and lignin changes in relation to vitrification of carnation tissues cultured in vitro.-J. Plant Physiol. 118: 41-48, 1985. Go to original source...
    20. Kevers, C., Hausman, J.F., Faivre-Rampant, O., Evers, D., Gaspar, Th.: Hormonal control of adventitious rooting: progress and questions.-J. appl. Bot. 71: 71-79, 1997.
    21. Lagrimini, L.M.: Altered phenotypes in plants transformed with chimeric tobacco peroxidase genes.-In: Lobarzewski, J., Greppin, H., Penel, C., Gaspar, T. (ed.): Biochemical, Molecular and Physiological Aspects of Plant Peroxidases. Pp. 59-67. University of Geneva, Geneva 1991.
    22. Letouzé, R.: Croissance du bourgeon axillairè chez Salix babylonica L. et activité phenylalanine ammonia lyase.-Planta 123: 155-162, 1975. Go to original source...
    23. Moncousin, C., Favre, J.M., Gaspar, Th.: Changes in peroxidase activity and endogenous IAA levels during adventitious rooting in vine cuttings.-In: Kutáček, M., Bandurski, R.S., Krekule, J. (ed.): Physiology and Biochemistry of Auxins in Plants. Pp. 331-337. Academia, Praha 1988.
    24. Murashige, T., Skoog, F.: A revised medium for rapid growth and bio assays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    25. Nordström, A.C., Eliasson, L.: Levels of endogenous indole-3-acetic acid and indole-3-acetylaspartic acid during adventitious root formation in pea cuttings.-Physiol. Plant. 82: 599-605, 1991. Go to original source...
    26. Pedreno, M.A., Ferrer, M.A., Gaspar, T., Munoz, R., Ros Barcelo, A.: The polyfunctionality of cell wall peroxidases avoids the necessity of an independent H2O2-generating system for phenolic coupling in the cell wall.-Plant Peroxidase Newslett. 5: 3-8, 1995.
    27. Reverberi, M., Falasca, G., Lauri, P., Caboni, E., Altamura, M.M.: Indoleacetic acid induces xylem formation instead of rooting in walnut (Juglans regia L.) microcuttings.-Plant Biosyst. 135: 71-77, 2001. Go to original source...
    28. Richardson, A., McDougall, G.J.: A laccase-type polyphenol oxidase from lignifying xylem of tobacco.-Phytochemistry 44: 229-235, 1997. Go to original source...
    29. Ripetti, V., Kevers, C., Gaspar, Th.: Two successive media for the rooting of walnut shoots in vitro. Changes in peroxidase activity and ethylene production.-Adv. hort. Sci. 8: 29-32, 1994.
    30. Scaltsoyiannes, A., Tsoulpha, P., Panetsos, K.P., Moulalis, D.: Effect of genotype on micropropagation of walnut trees (Juglans regia).-Silvae genet. 46: 326-332, 1998.
    31. Spector, T.: Refinement of Coomassie blue method of protein quantitation.-Anal. Biochem. 86: 142-146, 1978. Go to original source...
    32. Whetten, R.W., Sederoff, R.R.: Lignin biosynthesis.-Plant Cell 7: 1001-1013, 1995. Go to original source...

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