biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:7-11, 2003 | DOI: 10.1023/A:1027312410957

Somatic Embryo Formation on Mature Abies alba × Abies cephalonica Zygotic Embryo Explants

T. Salaj1, J. Salaj1
1 Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra 1, Slovak Republic

Somatic embryogenesis was achieved from mature embryos excised from stored hybrid Abies alba × Abies cephalonica seeds. Embryogenic tissue formation occurred on SH medium supplemented with 1 mg dm-3 benzyladenine. The formation of embryogenic tissue was influenced by the time of storage of seeds. Initiation frequencies 27.2 - 29.0 % were obtained in embryos isolated from 6 month and 1 year stored seeds. Embryos excised from 4-year stored seeds showed no response. Embryogenic structures appeared on the surface of hypocotyl. They originated without previous callus formation. Embryogenic tissues were maintained in long-term cultures. After maturation treatment cotyledonary somatic embryos developed and germinated in small plantlets.

Keywords: benzyladenine; conifers; hybrid fir; histology
Subjects: Abies alba × Abies cephalonica; cytokinins; fir, somatic embryogenegis; in vitro culture, nutrient media modification; in vitro culture, somatic embryogenegis; somatic embryo formation, mature zygotic embryo explants

Published: July 1, 2003  Show citation

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Salaj, T., & Salaj, J. (2003). Somatic Embryo Formation on Mature Abies alba × Abies cephalonica Zygotic Embryo Explants. Biologia plantarum, 46(1), 7-11. doi: 10.1023/A:1027312410957
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