biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:33-38, 2003 | DOI: 10.1023/A:1027320612774

Changes in the Content of Modified Nucleotides in Wheat rRNA during Greening

I. Parádi1, E. Páldi3, S. Rudnóy1, Z. Bratek1, G. Kovács2, I. Rácz1, D. Lásztity1
1 Department of Plant Physiology, Eötvös Loránd University, Budapest, Hungary
2 Department of Microbiology, Eötvös Loránd University, Pázmány Péter sétány 1/c, Budapest, Hungary
3 Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Hungary

The modified nucleotide content of the ribosomal RNAs in wheat is greatly influenced by light. The rRNAs of etiolated seedlings contain far fewer modified derivatives. The modified nucleotide composition characteristic of green plants develops gradually as a result of irradiation. In the course of the experiments changes in the state of modification of 5.8S and 18S rRNAs were examined during the greening of etiolated wheat seedlings. Three types of minor nucleotides, O2'-methyladenosine, O2'-methylguanosine and pseudouridine were found in the 5.8S rRNA of green wheat leaves, none of which was detected in etiolated wheat. The minor nucleotides appeared in the 5.8S rRNA only after 48 h irradiation. The sequences of 5.8S rDNA, TTS1, ITS2 and 18S rDNA were also determined and the presence of the hyper-modified nucleotide 1-methyl-3-(α-amino-α-carboxypropyl)-pseudouridine was detected in green wheat 18S rRNA. This minor component was not demonstrable in etiolated wheat 18S rRNA, but appeared after irradiation for 48 h.

Keywords: 5,8S rRNA; 18S rRNA; ITS1 and ITS2; hyper-modified pseudouridine
Subjects: growth analysis, plant development, biomass enhancement; hyper-modified pseudouridine; nucleotide, ribosomal RNAs; wheat, rRNA during greening

Published: July 1, 2003  Show citation

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Parádi, I., Páldi, E., Rudnóy, S., Bratek, Z., Kovács, G., Rácz, I., & Lásztity, D. (2003). Changes in the Content of Modified Nucleotides in Wheat rRNA during Greening. Biologia plantarum, 46(1), 33-38. doi: 10.1023/A:1027320612774
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    References

    1. Bachellerie, J.P., Cavaillé, J.: Guided ribose methylation of RNAs.-Trends biochem. Sci. 22: 257-261, 1997. Go to original source...
    2. Björk, G.R., Swensson, I.: Analysis of methylated constituents from RNA by thin-layer chromatography.-Biochem. biophys. Acta 138: 430-432, 1967. Go to original source...
    3. Bousquet-Antonelli, C., Henry, Y., Gélunge, J.P., Caisergues-Ferrer, M., Kiss, T.: A small nucleolar RNP protein is required for pseudouridylation of eukaryotic ribosomal RNAs.-EMBO J. 15: 4470-4476, 1997. Go to original source...
    4. Brand, R.C., Klootwijk, J., Planta, R.J., Maden, B.E.H.: Biosynthesis of hypermodified nucleotide in Saccharomyces carlsbergensis 17S and HeLa-cell 18S ribosomal ribonucleic acid.-Biochem. J. 169: 71-77, 1978. Go to original source...
    5. Cecchini, J.P., Miassod, R.: Studies on the methylation of cytoplasmic ribosomal RNA from cultured higher plant cells.-Eur. J. Biochem. 98: 203-214, 1979. Go to original source...
    6. Cornelis, P.: Use of high salt Sepharose 4B chromatography for the extraction of tRNA of plant tissues.-Plant Sci. Let. 11: 3-8, 1978. Go to original source...
    7. Gillam, S., Millward, S., Blew, D., Tigerstrom, M., Wimmer, E., Tener, C.M.: The separation of soluble ribonucleic acids on benzoylated diethylaminoethyl cellulose.-Biochemistry 6: 3043-3056, 1967. Go to original source...
    8. Kirby, K.S.: Isolation of nucleic acids with phenolic solvents.-In: Grossman, L., Moldave, K. (ed.): Methods in Enzymology. Vol. 12B. Pp. 87-99. Academic Press, New York-London 1968. Go to original source...
    9. Lásztity, D., Rácz, I., Király, I., Jakucs, E., Páldi, E.: Effect of light on the activity of the protein synthesising system in wheat sedlings.-Plant Sci. 77: 173-176, 1991. Go to original source...
    10. Lásztity, D., Rácz, I., Páldi E.: Effect of long periods of low temperature on protein synthesis activity in wheat seedlings.-Plant Sci. 149: 59-62, 1999. Go to original source...
    11. Loening, U.E.: The fractionation of high-molecular-weight ribonucleic acids by polyacrylamide-gel electrophoresis.-Biochem. J. 102: 251-257, 1967. Go to original source...
    12. Mackay, R.M., Spencer, D.F., Doolittle, W.F., Gray, M.W.: Nucleotide sequences of wheat embryo cytosol 5S and 5.8S ribosomal ribonucleic acids.-Eur. J. Biochem. 112: 561-576, 1980. Go to original source...
    13. Maden, B.E.H.: The numerous modified nucleotides in eukaryotic ribosomal RNA.-Progr. nucl. Acids Res. mol. Biol. 39: 242-303, 1990. Go to original source...
    14. Mathews, D.H., Sabina, J., Zuker, M., Turner, D.H.: Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.-J. mol. Biol. 288: 911-940, 1999. Go to original source...
    15. Murray, M.G., Thompson, W.F.: Rapid isolation of high-molecular-weight plant DNA.-Nucl. Acids Res. 8: 4321-4325, 1980. Go to original source...
    16. Rácz, I., Király, I., Lásztity, D.: Effect of light on the nucleotide composition of rRNA of wheat seedlings.-Planta 142: 263-267, 1978. Go to original source...
    17. Rácz, I., Juhász, Á., Király, I., Lásztity, D.: Changes in minor nucleotide content of 18S+26S rRNA of wheat seedlings during greening.-Physiol. vég. 21: 229-232, 1983.
    18. Saponara, A.G., Enger, M.D.: The isolation from ribonucleic acid of substituted uridines containing α-aminobutyrate moieties derived from methionine.-Biochim. biophys. Acta 349: 61-67, 1974. Go to original source...
    19. Saunders, G.W., Druehl, L.D.: Nucleotide sequences of the small subunit ribosomal RNA genes from selected Laminariales (Phaeophyta): implications for kelp evolution.-J. Phycol. 28: 544-549, 1992. Go to original source...
    20. Venema, J., Tollervey, D.: Ribosome synthesis in Saccharomyces cerevisiae.-Annu. Rev. Genet. 33: 261-311, 1999. Go to original source...
    21. White, T.J., Bruns, T.D., Lee, S., Taylor, J.W.: PCR-Protocols -A Guide to Methods and Applications. Part 3.-Academic Press, New York 1990.
    22. Zuker, M., Mathews, D.H., Turner, D.H.: Algorithms and thermodynamics for RNA secondary structure prediction.-In: Barciszewski, J., Clark, B.F.C. (ed.): A Practical Guide in RNA Biochemistry and Biotechnology. Pp. 11-43. Kluwer Academic Publishers, Dordrecht 1999. Go to original source...

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