biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:39-44, 2003 | DOI: 10.1023/A:1027372629612

Improved Performance of Transgenic Glycinebetaine-Accumulating Rice Plants under Drought Stress

W. Sawahel1
1 Vienna Biocenter, Institute of Microbiology and Genetics, Vienna, Austria

Plasmid DNA (pChlCOD), containing the selectable hygromycin phosphotransferase hpt gene for hygromycin B resistance and the Arthrobacter globiformis codA gene for choline oxidase which catalyzes the direct conversion of choline to glycinebetaine, was delivered into rice plants using Agrobacterium-mediated gene transfer via scutellum-derived calli. Southern, Northern and Western blot analyses demonstrated that the foreign gene had been transferred, integrated into rice chromosomal DNA and expressed. Drought test indicated that glycinebetaine acts as an osmoprotectant and its production in transgenic rice plant helped the cells to maintain osmotic potential and increased root growth, and thus enhanced the ability of the plants to tolerate water deficit

Keywords: Agrobacterium-mediated transformation; choline oxidase gene; Oryza sativa L.
Subjects: Agrobacterium-mediated tranformation; Agrobacterium tumefaciens; choline oxidase; genetic transformation; glycine betaine; Oryza sativa; osmotic potential, transgenic rice; transgenic plants; water stress, transgenic rice

Published: July 1, 2003  Show citation

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Sawahel, W. (2003). Improved Performance of Transgenic Glycinebetaine-Accumulating Rice Plants under Drought Stress. Biologia plantarum, 46(1), 39-44. doi: 10.1023/A:1027372629612
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