biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:53-59, 2003 | DOI: 10.1023/A:1027376730521

Free Amino Acid, Protein and Water Content Changes Associated with Seed Development in Araucaria angustifolia

L.V. Astarita1, E.I.S. Floh1, W. Handro1
1 Plant Cell Biology Laboratory, Department of Botany, Institute of Biosciences, University of São Paulo, IB-USP, São Paulo, Brazil

The free amino acid, protein, water and dry matter contents were determined during the seed development of Araucaria angustifolia. Soluble and insoluble proteins in the mature seed represent 4.2 % of the fresh matter. The embryonic axis stored the greatest amount of soluble proteins, while cotyledons both with the embryonic axis showed the largest quantities of insoluble proteins in the mature seed. The greatest concentration of free amino acids was detected during the stage when cotyledons start to develop. Glutamic acid, aspartic acid, alanine and serine were predominant in the whole seed while arginine, lysine and γ-aminobutyric acid were present in great amounts only in cotyledons and embryonic axis. Although megagametophyte was important as a source of free amino acids, it was not the major protein storage organ in the mature seed. In the embryogenetic process, the rise of cotyledons is closely related to physiological and biochemical changes.

Keywords: Brazilian pine; zygotic embryogenesis
Subjects: amino acids; Araucaria angustifolia; Brazilian pine, seed development; germination; proteins, changes with seed development; water content, changes with seed development; zygotic embryogenesis

Published: July 1, 2003  Show citation

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Astarita, L.V., Floh, E.I.S., & Handro, W. (2003). Free Amino Acid, Protein and Water Content Changes Associated with Seed Development in Araucaria angustifolia. Biologia plantarum, 46(1), 53-59. doi: 10.1023/A:1027376730521
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