Biologia plantarum 46:67-72, 2003 | DOI: 10.1023/A:1022310100557
Effect of Kinetin on Starch and Sucrose Metabolising Enzymes in Salt Stressed Chickpea Seedlings
- 1 Department of Biochemistry, Punjab Agricultural University, Punjab, India
Higher amylase activity in cotyledons of kinetin treated salt stressed (75 mM NaCl) chickpea (Cicer arietinum L. cv. PBG-1) seedlings, as compared to salt stressed seedlings was observed during a growth period of 7 d. The activities of acid and alkaline invertases were maximum in shoots and minimum in cotyledons under all conditions. The reduced shoot invertase activities under salt stress were enhanced by kinetin with a simultaneous increase in reducing sugar content. Kinetin increased the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) in both the cotyledons and shoots of stressed seedlings. Kinetin appears to increase the turnover of sucrose in the shoots of stressed seedlings.
Keywords: amylase; Cicer arietinum; invertase; sucrose synthase; sucrose phosphate synthase
Subjects: amylase; chickpea, salt stress, sucrose metabolism; Cicer arietinum; cytokinins; invertase; kinetin; salt stress, kinetin, sucrose metabolism; starch, salt stress, kinetin; sucrose metabolising enzymes; sucrose phosphate synthase, salt stress, kinetin; sucrose synthase
Prepublished online: March 1, 2003; Published: July 1, 2003 Show citation
References
- Amzallag, G.N., Lerner, H.R., Poljakoff-Mayber, A.: Exogenous ABA as a modulator of the response of sorghum to high salinity.-J. exp. Bot. 41: 1529-1534, 1990.
Go to original source... - Amzallag, G.N., Lerner, H.R., Poljakoff-Mayber, A.: Interaction between mineral nutrients, cytokinin and gibberellic acid during growth of sorghum at high salinity.-J. exp. Bot. 43: 81-87, 1992.
Go to original source... - Balibrea, M.E., Parra, M., Bolarín, M.C., Peréz-Alfocea, F.: Cytoplasmic sucrolytic activity controls tomato fruit growth under salinity.-Aust. J. Plant Physiol. 26: 561-568, 1999.
Go to original source... - Davis, B.D.: Regulation of α-amylase activity in bean stem tissues.-Plant Physiol. 74: 841-845, 1984.
Go to original source... - Dey, P.M.: Changes in forms of invertases during germination of mungbean seeds.-Phytochemistry 25: 51-53, 1986.
Go to original source... - Dubois, M., Gilles, K.A., Hamilton, J.K., Robers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substances.-Anal. Chem. 28: 350-356, 1956.
Go to original source... - Gascon, S., Lampen, J.O.: Purification of internal invertase of yeast.-J. biol. Chem. 243: 1567-1572, 1968.
Go to original source... - Gupta, A.K., Singh, J., Kaur, N., Singh, R.: Effect of polyethylene glycol induced water stress on uptake, interconversion and transport of sugars in chickpea seedlings.-Plant Physiol. Biochem. 31: 743-747, 1993.
- Kaur, S., Gupta, A.K., Kaur, N.: Gibberellin A3 reverses the effect of salt stress in chickpea (Cicer arietinum L.) seedlings by enhancing the amylase activity and mobilization of starch in cotyledons.-Plant Growth Regul. 26: 85-90, 1998.
Go to original source... - Kaur, S., Gupta, A.K., Kaur, N.: Effect of GA3, kinetin and indole acetic acid on carbohydrate metabolism in chickpea seedlings germinating under water stress.-Plant Growth Regul. 30: 61-70, 2000.
Go to original source... - Kerr, P.S., Torres, W.K., Huber, S.C.: Resolution of two molecular forms of sucrose phosphate synthase from maize, sorghum and spinach leaves.-Planta 170: 515-519, 1987.
Go to original source... - Kinraide, T.B.: Interaction among Ca+2, Na+ and K+ in salinity toxicity: quantitative resolution of multiple toxic and ameliorative effects.-J. exp. Bot. 50: 1495-1505, 1999.
Go to original source... - Kuiper, P.J.C., Kuiper, D., Schuit, J.: Root functioning under stress conditions-an introduction.-Plant Soil 111: 249-253, 1988.
Go to original source... - Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951.
Go to original source... - Miyagi, M., Oku, H., Chinen, I.: Purification and action pattern on soluble starch of α-amylase from sugarcane leaves.-Agr. biol. Chem. 54: 849-850, 1990.
Go to original source... - Munns, R., Schachtman, D.P., Candon, A.G.: The significance of a two phase growth response to salinity in wheat and barley.-Aust. J. Plant Physiol, 22: 561-569, 1995.
Go to original source... - Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962.
Go to original source... - Nelson, N.: A photometric adaptation of Somogyi method for the determination of glucose.-J. biol. Chem. 153: 375-380, 1944.
Go to original source... - Patric, J.W.: Sieve element unloading: cellular pathway, mechanism and control.-Physiol. Plant. 78: 298-308, 1990.
Go to original source... - Roy, D., Basu, N., Mark, R., Banerjee, S.K.: Changes in levels for endogenous gibberellin and abscisic acid in salt sensitive germinating rice by NaCl salinity.-Plant Physiol. Biochem. 22: 200-202, 1995.
- Soussi, M., Ocana, A., Lluch, A.: Effects of salt stress on growth, photosynthesis and nitrogen fixation in chick-pea (Cicer arietinum L.) cultivars under salt stress.-J. exp. Bot. 49: 1329-1337, 1998.
Go to original source... - Soussi, M., Lluch, C., Ocana, A.: Comparative study of nitrogen fixation and carbon metabolism in two chickpea (Cicer arietinum L.) cultivars under salt stress.-J. exp. Bot. 50: 1701-1708, 1999.
Go to original source...



