biologia plantarum

International journal on Plant Life established by Bohumil Nģmec in 1959

Biologia plantarum 46:79-84, 2003 | DOI: 10.1023/A:1022314201466

Response of Wild Type of Arabidopsis thaliana to Copper Stress

M. Wójcik1, A. Tukiendorf1
1 Department of Plant Physiology, Maria Curie-Sklodowska University, Akademicka 19, Lublin, Poland

Wild type of Arabidopsis thaliana plants were cultivated hydroponically in Hoagland and Arnon nutrient solution and treated with copper (5 - 100 µM) for 2, 4, 7 and 14 d. A progressive decrease of the root length and biomass was observed at increasing Cu concentration in the nutrient solution. Roots accumulated higher amounts of Cu than shoots at all Cu treatments. Changes of cell and chloroplast ultrastructure of Cu-treated plants were also observed. Cu application did not induce formation of Cu-phytochelatin complexes. Changes in glutathione and glutathione disulfide content observed in roots and shoots of Cu-treated plants suggest their participation in amelioration of metal-induced oxidative stress.

Keywords: copper toxicity and tolerance; glutathione; glutathione disulfide; phytochelatin
Subjects: Arabidopsis thaliana; copper toxicity tolerance; glutathione; phytochelatin, copper toxicity and tolerance

Prepublished online: March 1, 2003; Published: July 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Wójcik, M., & Tukiendorf, A. (2003). Response of Wild Type of Arabidopsis thaliana to Copper Stress. Biologia plantarum, 46(1), 79-84. doi: 10.1023/A:1022314201466
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Anderson, M.E.: Determination of glutathione and glutathione disulfide in biological samples.-Methods Enzymol. 113: 548-555, 1985. Go to original source...
    2. Angelov, M., Tsonev, T., Uzunova, A., Gaidardjieva, K.: Cu2+ effect upon photosynthesis, chloroplast structure, RNA, and protein synthesis of pea plants.-Photosynthetica 28: 341-350, 1993.
    3. Clemens, S.: Molecular mechanisms of plant metal tolerance and homeostasis.-Planta 212: 475-486, 2001. Go to original source...
    4. Cobbett, C.S.: A family of phytochelatin synthase genes from plant. fungal and animal species.-Trends Plant Sci. 4: 335-337, 1999. Go to original source...
    5. Cobbett, C.S.: Phytochelatins and their roles in heavy metal detoxification.-Plant Physiol. 123: 825-832, 2000. Go to original source...
    6. De Vos, C.H.R., Vonk, M.J., Schat, H.: Glutathione depletion due to copper-induced phytochelatin synthesis causes oxidative stress in Silene cucubalus.-Plant Physiol. 98: 853-858, 1992. Go to original source...
    7. Eleftheriou, E.P., Karataglis, S.: Ultrastructural and morphological characteristics of cultivated wheat growing on copper-polluted fields.-Bot. Acta 102: 134-140, 1989. Go to original source...
    8. Ernst, W.H.O., Verkleji, J.A.C., Schat, H.: Metal tolerance in plants.-Acta bot. neerl. 41: 229-248, 1992. Go to original source...
    9. Farga¹ovį, A.: Phytotoxic effect of Cd, Zn, Pb, Cu and Fe on Sinapis alba L. seedlings and their accumulation in roots and shoots.-Biol. Plant. 44: 471-473, 2001. Go to original source...
    10. Fernandes, J.C., Henriques, F.S.: Biochemical, physiological and structural effects of excess copper on plants.-Bot. Rev. 57: 246-273, 1991. Go to original source...
    11. Freedman, J.H., Ciriolo, M.R., Peisach, J.: The role of glutathione in copper metabolism and toxicity.-J. biol. Chem. 264: 5598-5605, 1989. Go to original source...
    12. Howden, R., Andersen, C.R., Goldsbrough, P.B., Cobbett, C.S.: A cadmium sensitive, glutathione-deficient mutant of Arabidopsis thaliana.-Plant Physiol. 107: 1067-1073, 1995a. Go to original source...
    13. Howden, R., Goldsbrough, P.B., Andersen, C.R., Cobbett, C.S.: Cadmium-sensitive, cadl mutants of Arabidopsis thaliana are phytochelatin deficient.-Plant Physiol. 107: 1059-1066, 1995b. Go to original source...
    14. Jiang, W., Liu, D., Liu, X.: Effects o copper on root growth, cell division and nucleolus of Zea mays.-Biol. Plant. 44: 105-109, 2001. Go to original source...
    15. Keltjens, W.G., Van Beusichem, M.L.: Phytochelatins as biomarkers for heavy metal toxicity in maize: single metal effects of copper and cadmium.-J. Plant Nutr. 21: 635-648, 1998. Go to original source...
    16. Leopold, I., Gunther, D., Smidt, J., Neumann, D.: Phytochelatins and heavy metal tolerance.-Phytochemistry 50: 1323-1328, 1999. Go to original source...
    17. Mehra, R.K., Mulchandani, P.: Glutathione-mediated transfer of Cu(I) into phytochelatins.-Biochem. J. 307: 697-705, 1995. Go to original source...
    18. Rauser, W.E.: Structure and function of metal chelators produced by plants.-Cell Biochem. Biophys. 31: 19-48, 1999. Go to original source...
    19. Reboredo, F., Henriques, F.: Some observations on the leaf ultrastructure of Halimione portulacoides (L.) Aellen grown in a medium containing copper.-J. Plant Physiol. 137: 717-722, 1991. Go to original source...
    20. Rennenberg, H., Brunold, C.: Significance of glutathione metabolism in plants under stress.-Progress Bot. 55: 142-155, 1994. Go to original source...
    21. Tukiendorf, A.: The role of glutathione in detoxification of cadmium and excess copper in spinach plants.-Acta Physiol. Plant. 15: 175-183, 1993.
    22. Tukiendorf, A., Rauser, W.E.: Changes in glutathione and phytochelatins in roots of maize seedlings exposed to cadmium.-Plant Sci. 70: 155-156, 1990. Go to original source...
    23. Van Vliet, C., Andersen, C.R., Cobbett, C.S.: Copper-sensitive mutant of Arabidopsis thaliana.-Plant Physiol. 109: 871-878, 1995. Go to original source...
    24. Zhou, J., Goldsbrough, P.B.: Structure, organization and expression of the metallothionein gene family in Arabidopsis.-Mol. gen. Genet. 248: 318-328, 1995. Go to original source...

    Return to the content