biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:95-100, 2003 | DOI: 10.1023/A:1022370319213

Influence of Antiviral Factor on Tobacco Mosaic Virus RNA Biosynthesis in Tobacco

M. Šindelářová1, L. Šindelář1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic

An antiviral factor (AVF) was separated by removing virus particles from extracts of tobacco mosaic virus (TMV) infected leaves using calcium phosphate gel and by column chromatography on DEAE cellulose. AVF was not found in the extracts from healthy plants. The AVF restricted the virus infectivity "in vivo" and significantly decreased the activity of key enzymes of metabolic pathways tending to the purine and pyrimidine nucleotides biosynthesis of viral- RNA (glucose-6-phosphate dehydrogenase, ribonucleases, phosphomonoesterase and phosphodiesterase). No inhibition of these enzymes was observed "in vitro" when the effect of different concentrations of AVF (0.25 - 250 µg cm-3) was examined.

Keywords: Nicotiana tabacum; AVF; protein; resistance; rRNA; virus RNA
Subjects: antiviral factor (AVF), effect on TMV; AVF see antiviral factor; glucose-6-phosphate dehydrogenase, antiviral factor; Nicotiana tabacum; phosphodiesterase, antiviral factor; phosphomonoesterase; proteins, antiviral factor; ribonucleases, antiviral factor; tobacco mosaic virus RNA biosynthesis; tobacco, TMV infection; virus RNA biosynthesis

Prepublished online: March 1, 2003; Published: July 1, 2003  Show citation

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Šindelářová, M., & Šindelář, L. (2003). Influence of Antiviral Factor on Tobacco Mosaic Virus RNA Biosynthesis in Tobacco. Biologia plantarum, 46(1), 95-100. doi: 10.1023/A:1022370319213
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