Biologia plantarum 46:117-120, 2003 | DOI: 10.1023/A:1027397302296
In vitro Micropropagation of a Medicinal Plant Species Sophora flavescens
- 1 The Institute of Cell Biology, Lanzhou University, Lanzhou, P.R. China
- 2 Medical College, Dalian University, Dalian, P.R. China
A micro-propagating system based on the young stem node segments of Sophora flavescens Ait. (Fabaceae) was established. Murashige and Skoog (MS) basal medium supplemented with 8.88 μM 6-benzyladenine (BA) plus 2.69 μM α-naphthalene acetic acid (NAA) and that with only 5.37 μM NAA were found the best in promoting proliferation of shoots and induction of root, respectively. Percentages of shoot induction and number of shoot per explant were up to 93.4 % and 4.2 and rooting rate to 82.4 %, respectively. The segments of the regenerated shoots could be continuously induced to reproduce new shoots through subculture on the same medium in 30-d intervals and still kept this activity after being subcultured for 6 generations. After the regenerated plantlets were transplanted to field, they grew well, showing no any visible abnormalities.
Keywords: auxins; multiple shoot formation; rooting
Subjects: auxins; cytokinins; in vitro culture, micropropagation; in vitro culture, nutrient media modification; multiple shoot formation; rooting in vitro; Sophora flavescens; thidiazuron
Published: July 1, 2003 Show citation
References
- Hu, J.F., Yang, Y.M.: [Artificial cultivation of Sophora flavescens Ait.]-Western China J. Pharm. Stud. 16: 62-63. 2001. [In Chinese.]
- Iturriaga, A., Jordan, M., Roveraro, C., Goreux, A.: In vitro culture of Sophora toromiro (Papilionaceae), an endangered species.-Plant Cell Tissue Organ Cult. 37: 201-204, 1994.
Go to original source... - Jain, P., Rashid, A.: Stimulation of shoot regeneration on Linum hypocotyl segments by thidiazuron and its response to light and calcium.-Biol. Plant. 44: 611-613, 2001. Go to original source...
- Jusaitis, M.: Micropropagation of adult Swainsona formosa Leguminosae: Papilionoideae.-In vitro cell. dev. Biol. Plant. 33: 213-220, 1997. Go to original source...
- Kaneda, Y., Tabei, Y., Nishimura, K., Akihama, T., Kitamura, K.: Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybeans [Glycine max (L.) Merr.].-Plant Cell Rep. 17: 8-12, 1997. Go to original source...
- Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
- Ryu, S.Y., Choi, S.U., Kim, S.K., No, Z.S., Lee, C.O., Ahn, J.W., Kim, S.H.: In vitro antitumour activity of flavonoids from Sophora flavescens.-Phytotherapy Res. 11: 51-53. 1997. Go to original source...
- Saritha, K.V., Prakash, E., Ramamurthy, N., Naidu, C.V.: Micropropagation of Spilanthes acmella Murr.-Biol. Plant. 45: 581-584, 2002. Go to original source...
- Srivastava, K., Tiwari, K.N., Singh, R., Singh, B.D., Jaiswal, H.K.: Shoot regeneration from immature cotyledons of Cicer arietinum.-Biol. Plant. 44: 333-337, 2001. Go to original source...
- Wang, S.Z., Tiang, X.T., Luo, X.F.: [Tissue culture of the gigas type and tetraploid clone of black locust.]-Plant physiol. Commun. sin. 35: 204-205, 1999. [In Chinese.]
- Wang, Z.Z., Li, K.Q., Hao, L.F., Liu, Q.H., Jing, Z.P., Ma, L.F., Huang, T.: [Tissue culture and plantlet regeneration of Sophora japonica.]-Plant physiol. Commun. sin. 28: 131, 1992. [In Chinese.]
- Xu, H.S., Xu, J.L., Huang, X.L.: [Effect of TDZ on plant tissue culture.]-J. Guangxi Bot. sin. 1: 77-80, 1996. [In Chinese.]
- Yang, J., Hu, Z., Guo, G.Q., Zheng, G.C.: In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert.-Plant Cell Tissue Organ Cult. 66: 35-39, 2001. Go to original source...