biologia plantarum

Biologia plantarum 46:133-136, 2003 | DOI: 10.1023/A:1027305604113

Effect of Carbon Source on the Shoot Proliferation Potential of Epicotyl Explants of Syzygium cuminii

N. Jain¹, S.B. Babbar¹

¹ Department of Botany, University of Delhi, Delhi, India

Black plum (Syzygium cuminii) explants were grown in vitro on Murashige and Skoog medium. Among the various saccharides tested, the best caulogenic response was afforded by sucrose both in terms of explant response and shoot developing potential. Within monosaccharides, mannose was totally inhibitory as on the medium supplemented with this the shoot buds failed to develop, while, fructose and xylose completely inhibited the opening as well as the elongation of shoot buds. Glucose and galactose did not completely inhibit the caulogenic response. Among disaccharides, other than sucrose, maltose totally inhibited the elongation of the developed shoot buds while lactose supported it to a limited extent. Sugar alcohols, though not as good as sucrose, proved better sources of carbon and energy than the other tested sugars. Sucrose at concentration 4 % proved to be the best in developing 4.2 shoots per explant.

Keywords: black plum; caulogenesis; fructose; galactose; glucose; lactose; maltose; mannitol; mannose; Myrtaceae; sorbitol; sucrose; xylose

Subjects: black plum, saccharide sources, in vitro culture; carbon source, shoot proliferation in vitro culture; fructose source in in vitro culture; galactose source in in vitro culture; glucose source in in vitro culture; in vitro culture, nutrient media modification; in vitro culture, saccharide sources; lactose, source in in vitro culture; maltose, source in in vitro culture; mannitol, mannose, source in in vitro culture; saccharide sources, in vitro culture; sorbitol, source in in vitro culture; sucrose, source in in vitro culture; Syzygium cuminii; xylose, source in in vitro culture

Published: July 1, 2003  Show citation