biologia plantarum

Biologia plantarum 46:145-148, 2003 | DOI: 10.1023/A:1027361721860

Effect of 6-benzyladenine and Casein Hydrolysate on Micropropagation of Amorpha fruticosa

H.H. Gao¹, W. Li¹, J. Yang¹, Y. Wang¹, G.Q. Guo¹, G.C. Zheng¹

¹ Institute of Cell Biology, Lanzhou University, Lanzhou, Gansu, P.R. China

Using apical and axillary nodes as explants, a rapid and efficient method for propagation of Amorpha fruticosa L. has been developed. When grown on Murashige and Skoog (MS) medium supplemented with 8 mg dm^-3 benzyladenine, 100 % explants responded with 4.94 shoots per explant after 6-weeks culture, and explants taken from the in vitro proliferated shoots subsequently produced multiple shoots when cultured on the same medium. The addition of casein hydrolysate (200 mg dm^-3) enhanced the number of shoots up to 8.77 per subculture, and coconut milk was found to promote the shoot elongation and make them grow more vigorously, 82.53 % excised shoots were rooted on half-strength MS medium containing 2.0 mg dm^-3 indoleacetic acid after 3 weeks of incubation. After acclimatization, all of the rooted plantlets established in soil, exhibiting uniform morphological and growth characteristics.

Keywords: acclimatization; false indigo; growth regulators; in vitro cultivation; plant regeneration

Subjects: Amorpha fruticosa; auxins; casein hydrolysate, micropropagation; cytokinins; false indigo; in vitro culture, acclimation from in vitro to ex vitro culture; in vitro culture, micropropagation; in vitro culture, nutrient media modification; in vitro culture, regeneration, organogenesis

Published: July 1, 2003  Show citation