biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:161-166, 2003 | DOI: 10.1023/A:1022844720795

Growth and Water Relations of Paulownia fortunei Under Photomixotrophic and Photoautotrophic Conditions

P.S. Sha Valli Khan1, T. Kozai2, Q.T. Nguyen3, C. Kubota2, V. Dhawan1
1 Plant Tissue culture, Bioresources and Biotechnology Division, Tata Energy Research Institute, New Delhi-, India
2 Laboratory of Environmental Control Engineering, Department of Bioproduction Science, Faculty of Horticulture, Chiba University, Matsudo, Chiba, Japan
3 Laboratory of Plant Cell Technology, Institute of Tropical Biology, Ho Chi Minh City, Vietnam

The growth and water relations of Paulownia fortunei in photoautotrophic cultures (nutrient medium lacking sucrose and growth regulator) with CO2 enrichment (PWAH) or without CO2 enrichment (PWAL) were compared with those in photomixotrophic shoot (PWC; 30 g dm-3 sucrose and 0.3 mg dm-3 N6-benzyladenine) and root cultures (PWR; 0.3 mg dm-3 indole-3-butyric acid). The photoautotrophic and photomixotrophic cultures were incubated under photosynthetic photon flux 125 and 60 μmol m-2 s-1, respectively. 100 % sprouting and significantly higher number of shoots (1.6) were obtained with PWAH as compared to PWAL and PWC. PWAH and PWAL stimulated spontaneous rooting from the cut end of axillary shoots. In PWAH, 84 % of shoots rooted with an average of 5.9 roots per shoot and 4.0 cm of root length in 21 d. Rooting of photomixotrophic shoot cultures were stimulated by an auxin treatment. In this case, 98.3 % of shoots were rooted with an average of 4.6 roots per shoot and 1.9 cm length. A microscopic observation on leaf abaxial surface prints from photomixotrophic shoot and root cultures showed widely open (6 - 8 μm) spherical stomata (12 - 14 μm) and from photoautotrophic cultures elliptical stomata (10 - 12 μm) with narrow openings (3 - 4 μm). Leaves from photomixo-trophic cultures had higher stomatal index as compared to photoautotrophic cultures. The rate of moisture loss from detached leaves was not varying significantly in different cultures.

Keywords: CO2 enrichment; micropropagation; stomata; water loss
Subjects: CO2 enhancement, in vitro culture, sugar supply; cytokinins; in vitro culture, micropropagation; in vitro culture, nutrient media modification; Paulownia fortunei; stomata opening, in vitro culture, sugar and CO2 supply; water loss, in vitro culture, sugar and CO2 supply

Prepublished online: March 1, 2003; Published: September 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Sha Valli Khan, P.S., Kozai, T., Nguyen, Q.T., Kubota, C., & Dhawan, V. (2003). Growth and Water Relations of Paulownia fortunei Under Photomixotrophic and Photoautotrophic Conditions. Biologia plantarum, 46(2), 161-166. doi: 10.1023/A:1022844720795
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bergmann, B.A., Moon, H.K.: In vitro adventitious shoot production in Paulownia.-Plant Cell Tissue Organ Cult. 16: 315-319, 1997. Go to original source...
    2. Fila, G., Ghashghaie, J., Hoarau, J., Cornic, G.: Photosynthesis, leaf conductance and water relations of in vitro cultured grapevine rootstock in relation to acclimatization.-Physiol. Plant. 102: 411-418, 1998. Go to original source...
    3. Heo, J., Kozai, T.: Forced ventilation micropropagation system for enhancing photosynthesis, growth and development of Sweet potato plantlets.-Environ. Control Biol. 37: 83-92, 1999. Go to original source...
    4. Imelda, M., Kubota, C., Kozai, T.: Photoautotrophic micropropagation of Acacia plantlets.-Jap. J. trop. Agr. 42: 29-30, 1998.
    5. Kirdmanee, C., Kitaya, Y., Kozai, T.: Effect of CO2 enrichment and supporting material on growth, photosynthesis and water potential of Eucalyptus shoots per plantlets cultures photoautotrophically in vitro.-In Vitro cell. dev. Biol. Plant 31: 144-149, 1995. Go to original source...
    6. Kozai, T.: Micropropagation under photoautotrophic conditions.-In: Debergh, P.C., Zimmerman, R.H. (ed.): Micropropagation: Technology and Application. Pp. 447-469. Kluwer Academic Publishers, Dordrecht 1991. Go to original source...
    7. Kozai, T., Fujiwara, K., Watanabe, I.: Fundamental studies on environments in plant tissue culture vessels. (2) Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers.-J. Agr. Meteorol. 42: 119-127, 1986. Go to original source...
    8. Kubota, C., Tajuddin, T., Kozai, T.: Photoautotrophic micropropagation of mangosteen plantlets.-Jap. J. trop. Agr. 42: 31-32, 1998. Go to original source...
    9. Long, R.D.: Photoautotrophic micropropagation-a strategy for contamination control.-In: Cassells, A.C. (ed.): Pathogen and Microbial Contamination Management in Micropropagation. Pp. 267-278. Kluwer Academic Publishers, Dordrecht 1997. Go to original source...
    10. Lucchesini, M., Mensuali-Sodi, A., Massai, R., Gucci, R.: Development of autotrophy and tolerance to acclimatisation of Myrtus communis transplants cultured in vitro under different aeration.-Biol. Plant. 44: 167-174, 2001. Go to original source...
    11. Murashige, T., Skoog, F.: A revised medium for growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 437-497, 1962. Go to original source...
    12. Nguyen, Q.T., Kozai, T., Niu, G., Uyen, V.N.: Photosynthetic characteristics of coffee (Coffee arabusta) plantlets in vitro in response to different CO2 concentrations and light intensities.-Plant Cell Tissue Organ Cult. 55: 133-139, 1999. Go to original source...
    13. Sampson, J.: A method of replacing dry or moist surfaces for examination by light microscopes.-Nature 191: 932, 1961. Go to original source...
    14. Serret, M.D., Trillas. M.L, Matas, J., Araus, J.L.: Development of photoautotrophy and photoinhibition of Gardenia jasmonides plantlets during micropropagation.-Plant Cell Tissue Organ Cult. 45: 1-16, 1996. Go to original source...
    15. Serret, M.D., Trillas, M.L, Matas, J., Araus, J.L.: The effect of different closure types, light and sucrose concentration carbon isotope composition and growth of Gardenia jasmonides plantlets during micropropagation and subsequent acclimation ex vitro.-Plant Cell Tissue Organ Cult. 47: 217-230, 1997. Go to original source...
    16. Sha Valli Khan, P.S., Evers, D., Hausman, J.F.: Stomatal characteristics and water relations of Quercus robur NL 100 in relation to acclimatization.-Silvae Genet. 48: 83-87, 1999.
    17. Sha Valli Khan, P.S., Rao, K.R., Hausman, J.F.: Clonal multiplication of Syzygium alternifolium (Wight.) Walp., through mature nodal segments.-Silvae Genet. 48: 45-50, 1999.
    18. Singh, C., Arora, Y.K.: Paulownia-the tree of the future.-Indian Forest. 49: 20-21, 1999.
    19. Vinterhalter, B., Vinterhalter, D., Neąković, M.: Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L.-Biol. Plant. 44: 185-188, 2001. Go to original source...
    20. Voleniková, M., Tichá, I.: Insertion profiles in stomatal density and sizes in Nicotiana tabacum L. plantlets.-Biol. Plant. 44: 161-16, 2001. Go to original source...
    21. Zobayed, S.M.A., Afreen-Zobayed, F., Kubota, C., Kozai, T.: Stomatal characteristics and leaf anatomy of potato plantlets cultured in vitro under photoautotrophic and photomixotrophic conditions.-In Vitro cell dev. Biol. Plant 35: 183-188, 1999. Go to original source...
    22. Zobayed, S.M.A., Afreen-Zobayed, F., Kubota, C., Kozai, T.: Mass propagation of Eucalvptus camadulensis in a scaled-up vessel under in vitro photoautotrophic condition.-Ann. Bot. 85: 587-592, 2000. Go to original source...

    Return to the content