biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:161-166, 2003 | DOI: 10.1023/B:BIOP.0000022245.64929.8b

Extracellular Matrix in Early Stages of Direct Somatic Embryogenesis in Leaves of Drosera spathulata

M. Bobák1, J. Šamaj3, E. Hlinková2, A. Hlavačka1, M. Ovečka4
1 Department of Plant Physiology, Comenius University, Bratislava, Slovak Republic
2 Department of Genetics, Comenius University, 15 Bratislava, Slovak Republic
3 Institute of Plant Genetics, Slovak Academy of Sciences, Nitra, Slovak Republic
4 Institute of Botany, Slovak Academy of Sciences, D, Bratislava, Slovak Republic

Leaves from mature in vitro grown plants of Drosera spathulata Labill. regenerated new plantlets on solid induction medium in light. Especially vascular sheath parenchyma cells located close to basal part of tentacule showed high embryogenic potential. Proembryoids arrising from the tentacule base part were visible by scanning electron microscopy. Their surface cells were linked and covered with thin external, fibrilar network representing an extracellular matrix (ECM). Proembryogenic surface cells were later connected by coarse strands of fibrils. Young protoderm was formed arround globular embryoids and its cells were characterized by "brain-like" surface structure. However, the surface of fully developed protodermal cells was practicaly smooth and cells were stick to each other very tightly in torpedo and cotyledonary shaped embryoids. The presence of ECM was typical only for somatic proembryos and globular embryos. The ECM network was never observed on the surface of heart and torpedo shaped embryos.

Keywords: benzylaminopurine; cell surface; 2,4-dichlorophenophenoxyacetic acid
Subjects: cytokinins; Drosera spathulata; extracellular matrix; in vitro culture, micropropagation; in vitro culture, nutrient media modification; in vitro culture, somatic embryogenegis

Published: September 1, 2003  Show citation

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Bobák, M., Šamaj, J., Hlinková, E., Hlavačka, A., & Ovečka, M. (2003). Extracellular Matrix in Early Stages of Direct Somatic Embryogenesis in Leaves of Drosera spathulata. Biologia plantarum, 46(2), 161-166. doi: 10.1023/B:BIOP.0000022245.64929.8b
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