biologia plantarum

Biologia plantarum 46:167-172, 2003 | DOI: 10.1023/B:BIOP.0000022246.09161.63

CO₂ Dynamics and Growth in Photoautotrophic and Photomixotrophic Apple Cultures

S. Morini¹, M. Melai¹

¹ Dipartimento di Coltivazione e Difesa delle Specie Legnose "G. Scaramuzzi", Sezione Coltivazioni Arboree, Pisa University, Pisa, Italy

The daily dynamics of CO₂ concentration in the culture vessels and the photoautotrophic or photomixotrophic growth capacity of apple (Malus pumila hybrid MM 106 paradisiaca× Northern Spy) cultures were studied. The photoautotrophic cultures were grown on a sugar-free growth medium and submitted (0S+CO₂) or not (0S-CO₂) to periodic injections of exogenous CO₂. The photomixotrophic cultures were grown in the presence of 30 g dm^-3 sucrose, with (30S+CO₂) or without (30S-CO₂) CO₂ enrichment. The photosynthetic photon flux density applied was of 210 ± 5 μmol m^-2s^-1. In the 0S-CO₂ treatment, CO₂ showed rather uniform and narrow light-dark fluctuations throughout the culturing cycle. In the 30S-CO₂ treatment, the daily ratio between CO₂ produced during the dark period and that uptaken during the following light period, was almost always above 1 with the only exception of a few days (from the 5^th to the 9^th day) when the amount of photosynthesised CO₂ was equal to or higher than that produced during dark respiration. The 0S+CO₂ cultures needed to be enriched all days with exogenous CO₂ to avoid periods of gas deficiency while in 30S+CO₂ the CO₂ injected the first culturing day was uptaken over 5 d; thereafter, daily injections were necessary. Culture fresh and dry mass, number of newly formed shoots and number of nodes per shoot in 0S+CO₂ treatment did not statistically differ from the values obtained with 30S-CO₂. The highest growth was observed in 30S+CO₂ treatment. The increase in culture fresh mass due to 1 μmol of CO₂ added to the culture vessels was 1.54 and 1.36 mg for 30S and 0S respectively, while in terms of dry mass the increase was about 2.5 times higher in the sugar-enriched treatment. CO₂ enrichment accounted for 77.3 % and 21.2 % of the final fresh mass in 0S+CO₂ and 30S+CO₂, respectively.

Keywords: CO₂ enrichment; in vitro culture; Malus pumila. photosynthetic photon flux density; sucrose

Subjects: CO₂ enrichment; in vitro culture, CO₂ concentration changes inside cultivation vessel; in vitro culture, saccharide sources; Malus pumila; mixotrophy; photon flux density, photoautotrophic and photomixotrophic culture; saccharide sources, in vitro culture; sucrose, source in in vitro culture

Published: September 1, 2003  Show citation