biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:179-183, 2003 | DOI: 10.1023/B:BIOP.0000022248.62869.c7

Antioxidant Enzyme Activities during in vitro Morphogenesis of Gladiolus and the Effect of Application of Antioxidants on Plant Regeneration

S. Dutta Gupta1,*, S. Datta1
1 Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur, India

Activity of antioxidant enzymes was evaluated during somatic embryogenesis and shoot organogenesis from cultured leaf segments of Gladiolus hybridus Hort. The effect of exogenous antioxidants on somatic embryogenesis and shoot organogenesis has also been monitored. Activity of superoxide dismutase (SOD) gradually increased during somatic embryogenesis. while activities of catalase (CAT) and peroxidase (POX) decreased. In contrast, increase in CAT and POX activity and a concomitant decrease in SOD activity were noted during shoot organogenesis. Exogenous application of antioxidants such as glutathione (GSH), α-tocopherol and ascorbate (AA) inhibited somatic embryogenesis but stimulated shoot organogenesis. The frequency of somatic embryogenesis increased with the addition of H2O2. However, H2O2 inhibited shoot organogenesis.

Keywords: catalase; Gladiolus hybridus; peroxidase; reactive oxygen species; shoot organogenesis; somatic embryogenesis; superoxide dismutase
Subjects: antioxidants; catalase; Gladiolus hybridus; gladiolus, morphogenesis in vitro; in vitro culture, morphogenesis, antioxidants; in vitro culture, somatic embryogenegis; morphogenesis in vitro, antioxidants; peroxidases; reactive oxygen species (ROS); shoot organogenesis, antioxidants; superoxide dismutase

Published: September 1, 2003  Show citation

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Dutta Gupta, S., & Datta, S. (2003). Antioxidant Enzyme Activities during in vitro Morphogenesis of Gladiolus and the Effect of Application of Antioxidants on Plant Regeneration. Biologia plantarum, 46(2), 179-183. doi: 10.1023/B:BIOP.0000022248.62869.c7
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