biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:211-215, 2003 | DOI: 10.1023/A:1022846526246

Development of Molecular Cytogenetics and Physical Mapping of Ribosomal RNA Genes in Lupinus

B. Naganowska1, J. Doležel2, W.K. Święcicki1
1 Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland
2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic

Identification of individual chromosomes in Lupinus is not possible due to gradient in size and similar morphology. To overcome this problem, molecular cytogenetics was developed for Lupinus. As an initial step in karyotype analysis, fluorescent in situ hybridization (FISH) was performed to determine genomic distribution of rRNA genes in L. hispanicus, L. luteus and L. × hispanicoluteus. It was found that all three diploid species posses two chromosome pairs carrying 18S-5.8S-25S rDNA and one chromosome pair carrying 5S rDNA. The use of probes for rDNA permitted unambiguous identification of three different pairs of chromosomes and revealed conservation of the number of rDNA loci among the three species. The study represents the first step in physical mapping of Lupinus genome through FISH by providing distinct chromosome landmarks.

Keywords: fluorescent in situ hybridization; Lupinus hispanicus; L. luteus; L. × hispanicoluteus; rRNA genes
Subjects: fluorescent in situ hybridisation, karyotype analysis; Lupinus hispanicoluteus; Lupinus hispanicus; Lupinus luteus; ribosomal RNA; ribosomal RNA genes, genomic distribution

Prepublished online: March 1, 2003; Published: September 1, 2003  Show citation

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Naganowska, B., Doležel, J., & Święcicki, W.K. (2003). Development of Molecular Cytogenetics and Physical Mapping of Ribosomal RNA Genes in Lupinus. Biologia plantarum, 46(2), 211-215. doi: 10.1023/A:1022846526246
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    References

    1. Adachi, J., Watanabe, K., Fukui, K., Ohmido, N., Kosuge, K.: Chromosomal location and reorganization of the 45S and 5S rDNA in the Brachyscome lineariloba complex (Asteraceae).-J. Plant Res. 110: 371-377, 1997. Go to original source...
    2. Ali, H.B., Meister, A., Schubert, I.: DNA content, rDNA loci, and DAPI bands reflect the phylogenetic distance between Lathyrus species.-Genome 43: 1027-1032, 2000. Go to original source...
    3. Atkins, C.A., Smith, P.M.C., Gupta, S., Jones, M.G.K., Caligari, P.D.S.: Genetics, cytology and biotechnology.-In: Gladstones, J.S., Atkins, C.A., Hamblin, J. (ed.): Lupins as Crop Plants: Biology, Production and Utilization. Pp. 67-92. CAB International, Wallingford-New York 1998.
    4. Calderini, O., Pupilli, F., Cluster, P.D., Mariani, A., Arcioni, S.: Cytological studies of the nucleolus organizing regions in the Medicago complex: sativa-coerulea-falcata.-Genome 39: 914-920, 1996. Go to original source...
    5. Cheng, Z., Presting, G.G., Buell, C.R., Wing, R.A., Jiang, J.: High-resolution pachytene chromosome mapping of bacterial artificial chromosomes anchored by genetic markers reveals the centromere location and the distribution of genetic recombination along chromosome 10 of rice.-Genetics 157: 1749-1757, 2001. Go to original source...
    6. Cuadrado, A., Schwarzacher, T.: The chromosomal organization of simple repeats in wheat and rye genomes.-Chromosoma 107: 587-594, 1998. Go to original source...
    7. Doleželová, M., Valárik, M., Swennen, R., Horry, J.P., Doležel, J.: Physical mapping of the 18S-25S and 5S ribosomal RNA genes in diploid bananas.-Biol. Plant. 41: 497-505, 1998. Go to original source...
    8. Dong, F., Song, J., Naess, S.K., Helgeson, J.P., Gebhardt, C., Jiang, J.: Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato.-Theor. appl. Genet. 101: 1001-1007, 2000. Go to original source...
    9. Fuchs, J., Kühne, M., Schubert, I.: Assignment of linkage groups to pea chromosomes after karyotyping and gene mapping by fluorescent in situ hybridization.-Chromosoma 107: 272-276, 1998. Go to original source...
    10. Gladstones, J.S.: Distribution, origin, taxonomy, history and importance.-In: Gladstones, J.S., Atkins, C.A., Hamblin, J. (ed.): Lupins as Crop Plants: Biology, Production and Utilization. Pp. 1-37. CAB International, Wallingford-New York 1998. Go to original source...
    11. Hanson, R.E., Islam-Faridi, M.N., Percival, E.A., Crane, C.F., Ji, Y., McKnight, T.D., Stelly, D.M., Price, H.J.: Distribution of 5S and 18S-28S rDNA loci in a tetraploid cotton (Gossypium hirsutum L. and its putative diploid ancestors.-Chromosoma 105: 55-61, 1996. Go to original source...
    12. Heslop-Harrison, J.S.: The molecular cytogenetics of plants.-J. Cell Sci. 100: 15-21, 1991. Go to original source...
    13. Kulikova, O., Gualtieri, G., Geurts, R., Kim, D.J., Cook, D., Huguet, T., de Jong, H., Fransz, P.F., Bisseling, T.: Integration of the FISH pachytene and genetic maps of Medicago truncatula.-Plant J. 27: 49-58, 2001. Go to original source...
    14. Maluszyńska, J., Heslop-Harrison, J.S.: Physical mapping of rDNA loci in Brassica species.-Genome 36: 774-781, 1993. Go to original source...
    15. Naganowska, B., Ladoń, D.: Chromosomes of Lupinus hispanicus subsp. hispanicus Boiss. et Reut., L. luteus L. and their hybrids.-J. appl. Genet. 41: 167-170, 2000.
    16. Nouzová, M., Kubaláková, M., Doleželová, M., Koblížková, A., Neumann, P., Doležel, J., Macas, J.: Cloning and characterization of new repetitive sequences in field bean (Vicia faba L.).-Ann. Bot. 83: 535-541, 1999. Go to original source...
    17. Obermayer, R., Święcicki, W.K., Greilhuber, J.: Flow cytometric detection of genome size in some Old World Lupinus species (Fabaceae).-Plant Biol. 1: 1-8, 1999. Go to original source...
    18. Ohmido, N., Fukui, K.: Cytological studies of African cultivated rice, Oryza glaberrima.-Theor. appl. Genet. 91: 212-217, 1995. Go to original source...
    19. Pickering, R.A., Malyshev, S., Künzel, G., Johnston, P.A., Korzun, V., Menke, M., Schubert, I.: Locating introgressions of Hordeum bulbosum chromatin within the H. vulgare genome.-Theor. appl. Genet. 100: 27-31, 2000. Go to original source...
    20. Raina, S.N., Mukai, Y.: Detection of a variable number of 18S-5.8S-26S and 5S ribosomal DNA loci by fluorescent in situ hybridization in diploid and tetraploid Arachis species.-Genome 42: 52-59, 1999. Go to original source...
    21. Rogers, S.O., Bendich, A.J.: Ribosomal RNA genes in plants: A variability in copy number and in the intergenic spacer.-Plant mol. Biol. 9: 509-520, 1987. Go to original source...
    22. Schmidt, T., Jung, C., Heslop-Harrison, J.S., Kleine, M.: Detection of alien chromatin conferring resistance to the beet cyst nematode (Heterodera schachtii Schm.) in cultivated beet (Beta vulgaris L.) using in situ hybridization.-Chromosome Res. 5: 186-1963, 1997.
    23. Święcicki, W., Święcicki, W.K., Nijaki, T.: Lupinus × hispanicoluteus - an interspecific hybrid of Old World lupins.-Acta Soc. Bot. Pol. 68: 217-220, 1999. Go to original source...
    24. Wolko, B., Weeden, N.F.: Estimation of Lupinus genome polyploidy on the basis of isozymic loci number.-Genet. Pol. 30: 165-171, 1989.
    25. Yakura, K., Tanifuji, S.: Molecular cloning and restriction analysis of EcoRI-fragments of Vicia faba rDNA.-Plant Cell Physiol. 24: 1327-1330, 1983.

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