biologia plantarum

International journal on Plant Life established by Bohumil Nģmec in 1959

Biologia plantarum 46:223-232, 2003 | DOI: 10.1023/A:1022850627155

Coiled Bodies in the Meristematic Cells of the Root of Lupinus luteus L.

B. Wróbel1, D.J. Smoliński1
1 Department of Cell Biology, Institute of General and Molecular Biology, N. Copernicus University, Toruń, Poland

The nature of nucleolar associate bodies in the meristematic cells of the root of Lupinus luteus L. was investigated using immunocytochemical methods, in situ hybridisation with light, confocal, and electron microscopy. The nuclear bodies of lupin proved to be structures containing fibrillarin and coilin, but devoid of rRNA and DNA, like animal coiled bodies (CBs). In lupin cells we have observed the occurrence of small nuclear ribonucleoprotein (snRNP) in the cytoplasm, in nucleoplasm, CBs and in nucleoli. This type of snRNP localisation pattern is in agreement with recently presented models of the small nuclear RNA cycle.

Keywords: Ag-NOR proteins; immunogold; nucleolus; ribosomal RNA; Sm proteins; snRNA; splicing
Subjects: coiled bodies, meristematic root cells; fibrillarin; immunogold; Lupinus luteus; meristematic cells, root; nucleolus; proteins, Ag-NOR; proteins, Sm, core spliceosomal proteins; ribosomal RNA; root, meristematic cells; smRNA; splicing

Prepublished online: March 1, 2003; Published: September 1, 2003  Show citation

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Wróbel, B., & Smoliński, D.J. (2003). Coiled Bodies in the Meristematic Cells of the Root of Lupinus luteus L. Biologia plantarum, 46(2), 223-232. doi: 10.1023/A:1022850627155
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    References

    1. Baserga, S.J., Yang, X.D., Steitz, J.A.: An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.-EMBO J. 10: 2645-2651, 1991. Go to original source...
    2. Beven, A.F., Lee, R., Razaz, M., Leader, D.J., Brown, J.W.S., Shaw, P.J.: The organization of ribosomal RNA processing correlates with the distribution of nucleolar snRNAs.-J. Cell Sci. 109: 1241-1251, 1996. Go to original source...
    3. Beven, A.F., Simpson, G.G., Brown, J.W.S., Show, P.J.: The organization of spliceosomal components in the nuclei of higher plants.-J. Cell Sci. 108: 509-518, 1995. Go to original source...
    4. Bohmann, K., Ferreira J.A., Santama, N., Weis, K., Lamond, A.I.: Molecular analysis of the coiled body.-J. Cell Biol. 19(Suppl.): 817-831, 1995. Go to original source...
    5. Boudonck, K., Dolan, L., Shaw, P.J.: The movement of coiled bodies visualized in living plant cells by the green fluorescent protein.-Mol. Biol. Cell 10: 2297-2307, 1999. Go to original source...
    6. Carmo-Fonseca, M., Ferreira, J., Lamond, A.: Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis-evidence that the coiled body is a kinetic nuclear structure.-J. Cell Biol. 120: 842-852, 1993. Go to original source...
    7. Chamberland, H., Lafontaine, J.G.: Localization of snRNP antigens in nucleolus-associated bodies: study of plant interphase nuclei by confocal and electron microscopy.-Chromosoma 102: 220-226, 1993. Go to original source...
    8. Chamberland, H., Spertini, D., Plante, M., Bellemare, G., Lafontaine, J.G.: Identification of coiled bodies in Brassica napus nuclei during embryogenesis and early germination.-Protoplasma 207: 106-113, 1999. Go to original source...
    9. Gulemetova, R., Chamberland, H., Gugg, S., Plante, M., Lafontaine, J.G.: Presence of small-nuclear-ribonucleoprotein-containing nuclear bodies in quiescent and early germinating Zea mays embryos.-Protoplasma 202: 192-201, 1998. Go to original source...
    10. Hadjiolov, A.A.: The Nucleolus and Ribosome Biogenesis.-Springer-Verlag, New York 1985. Go to original source...
    11. Huang, S., Spector, D.L.: Dynamic organization of pre-mRNA splicing factors.-J. cell. Biochem. 62: 191-197, 1996. Go to original source...
    12. Jiménez-Garcia, L.F., Segura-Valdez, M. de L., Ochs, R.L., Rothblum, L.I., Hannan, R., Spector, D.L.: Nucleogenesis: U3 snRNA-containing prenucleolar bodies move do sites of active pre-rRNA transcription after mitosis.-Mol. Biol. Cell 5: 955-966, 1994. Go to original source...
    13. Lafontaine, J.G., Chamberland, H.: Relationship of nucleolus-associated bodies with nucleolar organizer tracks in plant interphase nuclei (Pisum sativum).-Chromosoma 103: 545-553, 1995. Go to original source...
    14. Majewska-Sawka, A, Rodriguez-Garcia, M.I.: rRNA distribution during microspore development in anthers of Beta vulgaris L.: quantitative in situ hybridization analysis.-J. Cell Sci. 109: 859-866, 1996 Go to original source...
    15. Matera, G.: Nuclear bodies: multifaceted subdomains of the interchromatin space.-Trends cell Biol. 9: 302-309, 1999. Go to original source...
    16. Mattaj, I.W., Englmeier, L.: Nucleocytoplasmic transport: the soluble phase.-Annu. Rev. Biochem. 67: 265-306, 1998. Go to original source...
    17. Misteli, T.: Cell biology of transcription and pre-mRNA splicing: nuclear architecture meets nuclear function.-J. Cell Sci. 113: 1841-1849, 2000. Go to original source...
    18. Olmedilla, A., de Dios Alche, J., Rodriguez-Garcia M.C.: Nucleolar evolution and coiled bodies during meiotic prophase in Olea europaea: differential localization of nucleic acids.-Eur. J. Cell Biol. 74: 181-189, 1997.
    19. Ra¹ka, I.: Nuclear ultrastructures associated with the RNA synthesis and processing.-J. cell. Biochem. 59: 11-26, 1996. Go to original source...
    20. Ra¹ka, I., Andrade, L.E.C., Ochs, R.L., Chan, E.K.L., Chang, C.M., Roos, G., Tan, E.M.: Immunological and ultrastructural studies of the nuclear coiled body with autoimmune antibodies.-Exp. Cell Res. 195: 27-37, 1991. Go to original source...
    21. Sanchez-Pina, M.A., Kieft, H., Schel, J.H.N.: Immunocytochemical detection of non-histone nuclear antigens in cryosections of developing somatic embryos from Daucus carota L.-J. Cell Biol. 93: 615-622, 1989. Go to original source...
    22. Simpson, G.G., Filipowicz, W.: Splicing of precursors to mRNA in higher plants: mechanism, regulation and subnuclear organisation of the spliceosomal machinery.-Plant mol. Biol. 32: 1-41, 1996. Go to original source...
    23. Sleeman, J.E., Lamond, A.I.: Newly assembled snRNPs associate with coiled bodies before specles, suggesting a nuclear snRNP maturation pathway.-Curr. Biol. 9: 1065-1074, 1999. Go to original source...
    24. Smoliński, D.J., Górska-Brylass, A.: Coiled bodies - transcription dependent structures.-Folia histochem. cytobiol. 34 55, 1999a.
    25. Smoliński, D.J., Górska-Brylass, A.: Nuclear domains related to RNA maturation in meiocytes of the larch.-Pol. J. Pathol. 50: 216, 1999b.
    26. Spector, D.L.: Macromolecular domains within cell nucleus.-Annu. Rev. Cell Biol. 9: 265-315, 1993. Go to original source...
    27. Straatman, K.R., Schel, J.H.N.: Distribution of splicing proteins and putative coiled bodies during pollen development and androgenesis in Brassica napus L.-Protoplasma 216: 191-200, 2001. Go to original source...
    28. Thiry, M.: Highly sensitive immunodetection of DNA on sections with exogenous terminal deoxynucleotidyl transferase and non-isotopic nucleotide analogues.-J. Histochem. Cytochem. 40: 411-419, 1992. Go to original source...
    29. Williams, L.M., Jordan, E.G., Barlow, P.W.: The ultrastructure of nuclear bodies in interphase plant cell nuclei.-Protoplasma 118: 95-103, 1983. Go to original source...
    30. Wróbel, B., Stoynova, E.: The effect of kinetin on the nucleolar cycle in the meristematic root cells of Lupinus luteus L.-Cell Biol. int. Rep. 16: 985-992, 1992. Go to original source...

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