biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:243-251, 2003 | DOI: 10.1023/B:BIOP.0000022259.87594.96

Immunohistological Analysis of Chemically Induced Proteins in Sugar Beet

L. Burketová1, K. Štillerová2, M. Feltlová1, M. Šindelářová1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic
2 Czech University of Agriculture Prague, Prague 6, Czech Republic

Tissue-specific distribution of basic β-1,3-glucanase (Glu2), basic class II chitinase (Ch2), basic class IV chitinase (Ch4), and acidic class III chitinase (SE2) were examined both in leaves and roots of sugar beet treated with salicylic acid (SA), benzothiadiazole (BTH) and glycine betaine. Protein localization was monitored by immunohistological analysis using specific antibodies. BTH, SA as well as glycine betaine induced both Glu2 and chitinase isozymes in leaves and roots of treated plants. The enzymes were accumulated in extracellular space and cell walls. They were mostly deposited in parenchyma cells of leaves and cortex parenchyma and endodermis of roots. In leaf tissues, BTH and SA induced proteins more effectively than glycine betaine but the effect of glycine betaine in roots was as efficient as BTH and SA. Glycine betaine induced the formation of extracellular globuli containing Ch4. Induced proteins were spatially distributed over the whole plant regardless the site of the inducer application.

Keywords: benzothiadiazole; Beta vulgaris; chitinase; β-1,3-glucanase; glycine betaine; immunolocalization; salicylic acid; sugar beet
Subjects: benzothiadiazole; Beta vulgaris; chitinase; β-1,3-glucanase; glycine betaine; immunolocalization; protein localization, immunohistological analysis; salicylic acid; sugar beet, protein localization, immunohistological analysis

Published: September 1, 2003  Show citation

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Burketová, L., Štillerová, K., Feltlová, M., & Šindelářová, M. (2003). Immunohistological Analysis of Chemically Induced Proteins in Sugar Beet. Biologia plantarum, 46(2), 243-251. doi: 10.1023/B:BIOP.0000022259.87594.96
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