biologia plantarum

Biologia plantarum 46:281-284, 2003 | DOI: 10.1023/B:BIOP.0000022266.67097.3d

Morphometric Analysis of Chloroplasts of Cotton Leaf and Fruiting Organs

B.R. Bondada¹, D.M. Oosterhuis²

¹ Soil and Water Sciences, University of Florida, Fayetteville, USA

² Department of Crops, Soils, and Environmental Sciences, University of Arkansas, Fayetteville, USA

We examined morphological and ultrastructural differences in chloroplasts of cotton leaves and the fruiting organs, bract, and capsule wall to advance our understanding of their commonly observed differences in photosynthetic efficiency. Chloroplasts from leaves were large (7.1 μm long in cross section), lens shaped with a well developed membrane system differentiated into grana and stroma lamellae that occupied the large cross-sectional area (12.3 μm²) of the organelle. A few small plastoglobuli and starch grains were scattered in the stroma region. The bract chloroplasts were correlative of leaf chloroplasts in size (6.8 μm in length) and shape with the exception that the bract chloroplasts exhibited greater thylakoid number per granum (15.8) than the leaf chloroplasts (10.5). In contrast to leaf and bract, the capsule wall chloroplasts were smaller in size (4.3 μm) and cross sectional area (6.8 μm²) than either the leaf or bract. The most intriguing feature of the capsule wall chloroplasts was its domination by large starch granules (5.3 μm²) in the stroma which filled the whole chloroplast coercing the membrane system to move towards the periphery of the organelle. Grana number and thylakoids per granum were lowest in the capsule wall chloroplasts.

Keywords: bract; capsule wall; Gossypium hirsutum; grana; starch; thylakoids; transmission electron microscopy

Subjects: chloroplast, size, morphometry; cotton, morphometric analysis; fruiting organs, chloroplast, morphometry; Gossypium hirsutum; leaf, chloroplast, morphometry

Published: September 1, 2003  Show citation