biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:293-295, 2003 | DOI: 10.1023/B:BIOP.0000022269.30902.5c

Production of Doubled Haploids from Androgenic Embryoids and Plantlets of Tobacco

B. Bürün1, Ü. Emiroğlu2
1 Faculty of Science and Arts, Department of Biology, MugĞla University, MugĞla, Turkey
2 Faculty of Agriculture, Department of Field Crops, Ege University, İzmir, Turkey

In four experiments, the chromosome doubling effect of acenaphthene vapour was tested on androgenic embryoids and/or plantlets of tobacco. The maximum rate of plants with doubled chromosome numbers (41.3 % diploids plus 2.7 % tetraploids) was recorded when approx. 100 mg acenaphthene in small and sterilized cheese-cloth bags were hanged for 72 h, into the culture jars (6.0 × 11.5 cm), containing 4 - 6 androgenic plantlets newly transferred from culture tubes. Acenaphthene treatments performed at the earlier stages of culture resulted in lower percentages (from 0.0 to 6.3 %).

Keywords: acenaphthene; Nicotiana tabacum
Subjects: acenaphthene; haploid, doubled, from androgenic embryoids; Nicotiana tabacum; tobacco, androgenic embryoids

Published: September 1, 2003  Show citation

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Bürün, B., & Emiroğlu, Ü. (2003). Production of Doubled Haploids from Androgenic Embryoids and Plantlets of Tobacco. Biologia plantarum, 46(2), 293-295. doi: 10.1023/B:BIOP.0000022269.30902.5c
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