biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:297-300, 2003 | DOI: 10.1023/A:1022875332607

Somatic Embryogenesis or Shoot Formation Following High 2,4-D Pulse-Treatment of Mature Embryos of Paspalum scrobiculatum

Vikrant1, A. Rashid2
1 Department of Botany, University of Delhi, Delhi-, India
2 Department of Botany, University of Delhi, Delhi-, India

Mature zygotic embryos of Paspalum scrobiculatum L. cv. PSC 1 on MS or N6 nutrient medium supplemented with various concentrations of 2,4-D (4.5 - 22.5 µM) formed embryogenic callus, which differentiated into somatic embryos within 5 weeks of culture. The somatic embryos after transfer to hormone-free regeneration medium germinated and formed plantlets. Of the two nutrient formulations, N6 was relatively better than MS for somatic embryogenesis. A culture for 11 d on 100 µM 2,4-D was essential for the establishment of an embryogenic callus. Shorter duration, 4-d or 7-d culture on 2,4-D medium, supported some proliferation and subsequent differentiation into shoot-buds or multiple-shoots, in high-frequency cultures. This is first instance in monocots of a controlled regeneration response; either somatic embryogenesis or shoot formation.

Keywords: auxin; graminaceous plants; millet; organogenesis; regeneration
Subjects: auxins; in vitro culture, plant regeneration, organogenesis; in vitro culture, somatic embryogenesis; millet, somatic embryogenesis; Paspalum scrobiculatum; Poaceae, somatic embryogenesis

Prepublished online: March 1, 2003; Published: September 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Vikrant,, & Rashid, A. (2003). Somatic Embryogenesis or Shoot Formation Following High 2,4-D Pulse-Treatment of Mature Embryos of Paspalum scrobiculatum. Biologia plantarum, 46(2), 297-300. doi: 10.1023/A:1022875332607
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abdullah, R., Cocking, E.C., Thompson, JA.: Efficient plant regeneration from rice protoplasts through somatic embryogenesis.-Bio/Technology 4: 1087-1090, 1986. Go to original source...
    2. Arockiasamy, S., Prakash, S., Ignacimuthu, S.: High regenerative nature of Paspalum scrobiculatum L., an important millet crop.-Curr. Sci. 80: 496-498, 2001.
    3. Bhaskaran, S., Smith, R.H.: Regeneration in cereal tissue culture: a review.-Crop Sci. 30: 1328-1336, 1990. Go to original source...
    4. Bovo, O.A., Mroginski, L.A.: Somatic embryogenesis and plant regeneration from cultured mature and immature embryos of Paspalum notatum (Gramineae).-Plant Sci. 65: 217-223, 1989. Go to original source...
    5. Chu, C.C., Wang, C.C., Sun, C.S., Hsu, C., Yin, K.C., Chu, C.Y., Bi, F.Y.: Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources.-Sci. sin. 18: 659-668, 1975.
    6. Everett, N.P., Wach, M.J., Ashworth, D.J.: Biochemical markers of embryogenesis in tissue cultures of the maize inbred B73.-Plant Sci. 41: 133-140, 1985. Go to original source...
    7. Fransz, P.E., de Ruijter, N.C.A., Schel, J.H.N.: Isozymes as biochemical as cytochemical markers in embryogenic callus cultures of maize (Zea mays).-Plant Cell Rep. 218: 143-151, 1989. Go to original source...
    8. García, M.D., Molina, M.delC.: Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays × Zea mays ssp. parviglumis crosses.-Biol. Plant. 44: 497-501, 2001. Go to original source...
    9. Havrlentová, M., Faragó, J., Neąt'áková, M.: Regeneration of immature inflorescence of barley in vitro.-Biol. Plant. 44: 157-159, 2001. Go to original source...
    10. Lowe, K., Taylor, D.B., Ryan, P., Paterson, K.E.: Plant regeneration via organogenesis and embryogenesis in the maize inbred line B73.-Plant Sci. 41: 125-132, 1985. Go to original source...
    11. Morocz, S., Donn, G., Nemeth, J., Dudits, D.: An improved system to obtain fertile regenerants via maize protoplasts isolated from a highly embryogenic suspension culture.-Theor. appl. Genet. 80: 721-726, 1990. Go to original source...
    12. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    13. Nayak, P., Sen, S.K.: Plant regeneration through somatic embryogenesis from suspension cultures of a minor millet, Paspalum scrobiculatum.-Plant Cell Rep. 8: 296-299, 1989. Go to original source...
    14. Talwar, M., Rashid, A.: Factors affecting formation of somatic embryos and embryogenic callus from unemerged inflorescence of a graminaceous crop Pennisetum.-Ann. Bot. 66: 17-21, 1990. Go to original source...
    15. Vasil, I.K.: Somatic embryogenesis and its consequences in the Gramineae.-In: Henke, R.R., Hughes, K.W., Constantin, M.P., Hollaender, A. (ed.): Tissue Culture in Forestry and Agriculture. Pp. 31-47. Plenum Press, New York 1985. Go to original source...
    16. Vasil, V., Redway, F., Vasil, I.K.: Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.).-Bio/Technology 8: 429-434, 1990. Go to original source...
    17. Vikrant, Rashid, A.: Direct as well as indirect somatic embryogenesis from immature (unemerged) inflorescence of a minor millet Paspalum scrobiculatum L.-Euphytica 120: 167-172, 2001. Go to original source...
    18. Wang, D.Y., Vasil, I.K.: Somatic embryogenesis and plant regeneration from inflorescence segments of Pennisetum purpureum Schum. (napier or elephant grass).-Plant Sci. Lett. 25: 147-154, 1982. Go to original source...

    Return to the content