biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:301-304, 2003 | DOI: 10.1023/A:1022827416677

Primer Screening and Optimization for RAPD Analysis of Cashew

S. Samal1, G.R. Rout2, S. Nayak2, R.M. Nanda2, P.C. Lenka2, P. Das1
1 Department of Horticulture, Orissa University of Agriculture and Technology, Orissa, India
2 Plant Biotechnology Division, Regional Plant Resource Centre, Orissa, India

Primer screening and optimization for random amplified polymorphic DNA (RAPD) analysis of cashew (Anacardium occidentale L.) was investigated. Among four series (A, B, D and N) of 10-mer primers, A-series performed better amplification of fragments than other series. The maximum amplification fragments was obtained using OPA-02, OPA-03, OPA-09, OPB-06, OPB-10, OPD-03, OPD-05 and OPN-03 primers. The primers OPA-02 and OPN-03 produced maximum number of DNA fragments in Anacardium occidentale cv. H-320. Primers (OPB-08 and OPN-05 performed a least number of amplification fragments. RAPD profile also indicate that some primer did not produce good amplification. The primer OPA-02 amplified 12 number of polymorphic bands in 20 cultivars of cashew. Only one DNA fragment was produced in A. occidentale cv. Vridhachalam - 2 (M-44/3) by using the primer OPA-02.

Keywords: Anacardium occidentale; DNA amplification; polymerase chain reaction (PCR)
Subjects: Anacardium occidentale; cashew, RAPD; polymerase chain reaction (PCR); primer screening, RAPD optimization; random amplified polymorphic DNA (RAPD)

Prepublished online: March 1, 2003; Published: September 1, 2003  Show citation

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Samal, S., Rout, G.R., Nayak, S., Nanda, R.M., Lenka, P.C., & Das, P. (2003). Primer Screening and Optimization for RAPD Analysis of Cashew. Biologia plantarum, 46(2), 301-304. doi: 10.1023/A:1022827416677
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