Biologia plantarum 46:305-308, 2003 | DOI: 10.1023/A:1022879400747
Plant Micropropagation and Callus Induction of Some Annual Salsola Species
- 1 Department of General Botany, Institute of Experimental Biology, Adam Mickiewicz University, Poznań, Poland
- 2 Department of Desert Ecology, Samarkand Branch of Academy of Sciences, Samarkand, Uzbekistan
Micropropagated plants of two annual haloxerophytic Asiatic Salsola species (S. pestifer and S. lanata) were obtained from zygotic embryos cultured on Murashige and Skoog (MS) agar medium supplemented with 0.5 µM benzylamino-purine (BAP) and 0.3 µM indole-3-acetic acid (IAA) or with 0.5 µM 6 γ, γ-dimethylallylaminopurine and 0.3 μM IAA. The callus induction from shoot and leaf explants derived from plants propagated in vitro were obtained on MS agar medium with various concentration of auxins and cytokinins. The best medium for growth and proliferation of calluses of both studied species was MS medium containing 9.0 µM 2,4-dichlorophenoxyacetic acid. It was also determined that beginning of plant regeneration from callus of S. lanata was induced by 8.8 µM BAP.
Keywords: Chenopodiaceae; in vitro culture; Salsola lanata; Salsola pestifer
Subjects: auxins; cytokinins; in vitro culture, micropropagation; in vitro culture, nutrient media modification; Salsola lanata; Salsola pestifer
Prepublished online: March 1, 2003; Published: September 1, 2003 Show citation
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