biologia plantarum

Biologia plantarum 46:305-308, 2003 | DOI: 10.1023/B:BIOP.0000022272.39625.59

Plant Regeneration from Immature Embryo Cultures of Vigna unguiculata

P.-S. Choi^1,2, D.-Y. Cho³, W.-Y. Soh²

¹ Eugentech Inc./Bioventure, Korea Research Institute of Bioscience and Biotechnology, Tajeon, Korea

² Department of Biological Sciences, Chonbuk National University, Chonju, Korea

³ Department of Biology, Chonju Woosuk University, Chonju, Korea

Mature and immature cotyledon explants of cowpea were cultured on Murashige and Skoog's (MS) medium supplemented with 0.1 - 2.0 mg dm^-3 benzyladenine (BA). Shoot organogenesis was observed from the minimal greenish calli formed at proximal cutting edges of the immature cotyledon explants after 15 - 20 d of culture. Among whole immature zygotic embryo and seven explant types we tested, single whole cotyledon was suitable for shoot organogenesis. Nearly 67.5 % of the explant types produced adventitious shoots on MS medium containing with 1 mg dm^-3 BA, and the shoot number (10.1) per explant was higher than other explant types. From the histological studies, the shoot primordia originated from the procambial strands of immature cotyledon explants. When the shoots were transferred to 1/2 MS basal medium, formed roots within 20 d of culture. The rooted plants were subsequently transferred to the pots and to the field.

Keywords: cowpea; zygotic embryo explants; procambial strands; whole cotyledon; shoot organogenesis

Subjects: cowpea, cotyledon explants; embryo explant, immature; immature embryo culture, plant regeneration; in vitro culture, from immature embryo; in vitro culture, nutrient media modification; procambial strands; Vigna unguiculata; zygotic embryogenesis

Published: September 1, 2003  Show citation