biologia plantarum

Biologia plantarum 46:309-312, 2003 | DOI: 10.1023/B:BIOP.0000022273.55284.d6

Shoot Organogenesis from Immature Zygotic Embryo Cultures of Ginkgo biloba

P.-S. Choi^1,2, D.-Y. Cho³, W.-Y. Soh²

¹ Eugentech Inc./Bioventure, Korea Research Institute of Bioscience and Biotechnology, Tajeon, Korea

² Department of Biological Sciences, Chonbuk National University, Chonju, Korea

³ Department of Biology, Chonju Woosuk University, Chonju, Korea

Immature zygotic embryos were cultured on Murashige and Skoog's medium (MS) supplemented with various combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA), benzyladenine (BA) and zeatin or with various concentrations of 2,4-D alone. The maximum number (8 per embryo) of adventitious buds formed from cotyledons of heart stage embryos cultured on MS medium with 1 mg dm^-3 BA and 0.01 mg dm^-3 NAA. The adventitious buds originated from procambial strands of immature embryo cotyledons and then developed into adventitious bud primordia within 20 d. Adventitious buds transferred to hormone free MS medium grew into shoots, but did not produce plantlets because the shoots failed to root.

Keywords: adventitious shoot formation; benzyladenine; 2,4-dichlorophenoxyacetic acid; naphthaleneacetic acid; zeatin

Subjects: auxins; cytokinins; Ginkgo biloba

Published: September 1, 2003  Show citation