Biologia plantarum 46:313-316, 2003 | DOI: 10.1023/A:1022883501656
Stress-Induced Proteins in Parthenium argentatum Leaves
- 1 School of Life Sciences, Pondicherry University, Pondicherry, India
We have analyzed the stress-associated proteins in a high-rubber-yielding guayule (Parthenium argentatum Gray cv. 11591) leaves. Protein profiles in leaf fractions, resolved by SDS-PAGE and visualized by Coomassie Brilliant Blue staining, were different under various stresses. Changes in 25, 34 and 74 kDa polypeptides were noticed in response to low night temperature treatment while 24, 40, 47 and 81 kDa proteins responded to low irradiance. 23, 50, 75 and 82 kDa proteins were altered in response to drought stress. Certain proteins may play a significant role in the acquisition of tolerance in parenchyma cells of guayule leaves and might be useful markers to study adaptation in guayule plants.
Keywords: drought; guayule; irradiance; low night temperature; SDS-PAGE
Subjects: guayule, stress-induced proteins; Parthenium argentatum; photon flux density, polypeptide profile; proteins, drought-stress induced; stress-induced proteins, guayule; temperature, low, polypeptide profile; water stress, polypeptide profile
Prepublished online: March 1, 2003; Published: September 1, 2003 Show citation
References
- Backhaus, R.: Natural rubber from plants.-In: Kaplan, D.L. (ed.): Biopolymers from Renewable Resources. Pp. 323-354. Springer-Verlag, Heidelberg-Berlin 1998.
Go to original source... - Barathi, P., Sundar, D., Ramachandra Reddy, A.: Changes in mulberry leaf metabolism in response to water stress.-Biol. Plant. 44: 83-87, 2001.
Go to original source... - Bohnert, H.J., Nelson, D.E., Jensen, R.G.: Adaptations to environmental stresses.-Plant Cell 7: 1099-1111, 1995.
Go to original source... - Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding.-Anal. Biochem. 72: 248-254, 1976.
Go to original source... - Guy, C., Haskell, D., Li, Q.B.: Association of proteins with the stress 70 molecular chaperones at low temperature: Evidence for the existence of cold labile proteins in spinach.-Cryobiology 36: 301-314, 1998.
Go to original source... - Hernandez, L.D., Vierling, E.: Expression of low molecular weight heat-shock proteins under field conditions.-Plant Physiol. 101: 1209-1216, 1993.
Go to original source... - Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970.
Go to original source... - Magnard, J.L., Vergne, P., Dumas, C.: Complexity and genetic variability of heat-shock protein expression in isolated maize microspores.-Plant Physiol. 111: 1085-1096, 1996.
Go to original source... - Orr, A., Wagner, B.A., Howard, C.T., Schwartz, O.A.: Assay of plant proteins with bicinchoninic acid for high resolution two-dimensional polyacrylamide gel electrophoresis.-Plant Cell Rep. 7: 598-601, 1988.
Go to original source... - Pinedo, M.L., Hernández, G.F., Conde, R.D., Tognetti, J.A.: Effect of low temperature on the protein metabolism of wheat leaves.-Biol. Plant. 43: 363-367, 2000.
Go to original source... - Ramachandra Reddy, A., Das, V.S.R.: Enhanced rubber accumulation and rubber transferase activity in guayule under water stress.-J. Plant Physiol. 133: 152-155, 1988.
Go to original source... - Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular Cloning. A Laboratory Manual.-Cold Spring Harbour Laboratory Press, New York 1989.
- Shinozaki, K., Yamaguchi-Shinozaki, K.: Molecular responses to drought stress.-In: Shinozaki, K., Yamaguchi-Shinozaki, K. (ed.): Molecular Responses to Cold, Drought, Heat and Salt Stress in Higher Plants.-R.G. Landes Company, Austin 1999.
Go to original source... - Singla, S.L., Grover, A.: Detection and quantitation of a rapidly accumulating and predominant 104 kDa heat shock polypeptide in rice.-Plant Sci. 97: 23-30, 1994.
Go to original source... - Sundar, D., Ramachandra Reddy, A.: Low night temperature-induced changes in photosynthesis and rubber biosynthesis in guayule (Parthenium argentatum Gray).-Photosynthetica 38: 421-427, 2000.
Go to original source... - Sundar, D., Ramachandra Reddy, A.: Interactive influence of temperature and growth light intensity on rubber accumulation and rubber transferase activity in guayule (Parthenium argentatum Gray).-J. Plant Physiol. 158: 1291-1297, 2001.
Go to original source... - Vierling, E.: The roles of heat shock proteins in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 579-620, 1991.
Go to original source...



