biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:331-336, 2003 | DOI: 10.1023/A:1024305630919

In vitro Culture of Immature Embryos of Cytisus laburnum

F. Paolicchi1, P. Picciarelli1, R. Lorenzi2
1 Dipartimento di Biologia delle Piante Agrarie, Università di Pisa, Pisa, Italy
2 Dipartimento di Scienze Botaniche, Università di Pisa, Pisa, Italy

Immature embryos of Cytisus laburnum L. were cultivated in vitro and four culture media, different techniques of substrate preparation, sucrose concentration and the effect of suspensor removal were tested. The best results were obtained with N6 medium supplemented with 2 mg dm-3 glycine and set up using a double-layer culture system, in which the top layer had a higher osmotic potential than the bottom one. These conditions allowed normal embryogenic development in up to 45 % of early globular embryos, that were able to develop until a complete maturity. Osmotic potential and mineral nutrients of the medium demonstrated to be crucial for the successful culture and their effects were dependent on embryo age at the time of excision. The presence of an intact suspensor showed to be beneficial only for early globular embryos while older developmental stage embryos were not significantly affected.

Keywords: Golden chain; suspensor; zygotic embryogenesis
Subjects: Cytisus laburnum; in vitro culture, immature embrya; in vitro culture, nutrient media modification; osmotic potential, nutrient media; suspensor, embryo; zygotic embryogenesis

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

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Paolicchi, F., Picciarelli, P., & Lorenzi, R. (2003). In vitro Culture of Immature Embryos of Cytisus laburnum. Biologia plantarum, 46(3), 331-336. doi: 10.1023/A:1024305630919
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