biologia plantarum

Biologia plantarum 46:337-341, 2003 | DOI: 10.1023/A:1024357614990

Effect of Cytokinins and Cytokinin Antagonists on in vitro Cultured Gypsophila paniculata L.

V. Kapchina-Toteva¹, D. Stoyanova²

¹ Department of Plant Physiology, Faculty of Biology, University of Sofia, Sofia, Bulgaria

² Department of Botany, Faculty of Biology, University of Sofia, Sofia, Bulgaria

This study deals with the effects of two cytokinins [kinetin (Kin) and N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU)] and cytokinin antagonists [2-chloro-4-cyclobutyl-amino-6-ethylamino-1,3,5-triazine (ACK1) and N-(4-pyridyl)-O-(4-chlorophenyl)carbamate (ACK2)] in concentration of 1 μM on in vitro cultured Gypsophila. The application of Kin and CPPU stimulated bud opening and increased fresh and dry masses. Cytokinin antagonists reduced the number of sprouted buds and bud fresh and dry masses. In plants treated with CPPU the chloroplasts possessed well developed membrane system, which covered almost the entire chloroplasts volume. In ACK2 treated plants, the plastid apparatus in each cell was represented by two types of chloroplast in which the inner membrane system was differently organized. Cell wall adjacent chloroplasts possessed structure similar to the controls. In inner located chloroplasts part of thylakoids were semi-concentrically arranged and partially destructed.

Keywords: anticytokinins; axillary buds; chlorophyll a+b; chloroplast ultrastructure; in vitro propagation

Subjects: anticytokinins; axillary buds, cytokinins and anticytokinins; chlorophyll, cytokinins and anticytokinins; chloroplast ultrastructure, cytokinins and anticytokinins; cytokinin antagonists; cytokinins; Gypsophila paniculata; in vitro culture, micropropagation; kinetin; N-(4-pyridyl)-O-(4-chlorophenyl)carbamate; thylakoid, cytokinins and anticytokinins

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation