biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:343-347, 2003 | DOI: 10.1023/A:1024309731828

Propagation of Angelica archangelica Plants in an Air-Sparged Bioreactor from a Novel Embryogenic Cell Line, and their Production of Coumarins

M. Eeva1, T. Ojala1, P. Tammela2, B. Galambosi3, H. Vuorela1, R. Hiltunen1, K. Fagerstedt4, P. Vuorela2
1 Department of Pharmacy, Division of Pharmacognosy, University of Helsinki, Helsinki, Finland
2 Department of Pharmacy, DDTC, University of Helsinki, Helsinki, Finland
3 Ecological Production, Agricultural Research Centre of Finland, Mikkeli, Finland
4 Department of Bioscience, Division of Plant Physiology, University of Helsinki, Helsinki, Finland

A spontaneously embryogenic cell line of the coumarin producing angelica [Angelica archangelica (L.) subsp. archangelica] was established via callus formation from seedlings grown from sterilized seeds on semi-solid, hormone-free modified B5 medium. The cell line has retained its embryogenic capacity for 5 years. The highest coumarin production for the cell line after 3 weeks of cultivation was achieved in the medium containing 3.0 % sucrose. Jasmonic acid had no statistically significant effect on the biomass or coumarin production. The established embryogenic cell line could be stored using cryopreservation. Plantlets grown in an air-sparged bioreactor were transferred directly to soil and vermiculite, and 63 % of them grew to maturity through two growth seasons. The coumarin content in the regenerated plants was comparable to that in wild plants. Thus this cell line could be used for in vitro propagation.

Keywords: somatic embryogenesis; regeneration; preservation; coumarin analysis
Subjects: Angelica archangelica; bioreactor; coumarin; cryopreservation; in vitro culture, cryopreservation; in vitro culture, micropropagation; in vitro culture, somatic embryogenesis; in vitro culture, suspension culture; jasmonates, jasmonic acid

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

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Eeva, M., Ojala, T., Tammela, P., Galambosi, B., Vuorela, H., Hiltunen, R., Fagerstedt, K., & Vuorela, P. (2003). Propagation of Angelica archangelica Plants in an Air-Sparged Bioreactor from a Novel Embryogenic Cell Line, and their Production of Coumarins. Biologia plantarum, 46(3), 343-347. doi: 10.1023/A:1024309731828
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