biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:363-368, 2003 | DOI: 10.1023/A:1024369917715

The 26S Proteasome of the Resurrection Plant Tortula ruralis: Cloning and Characterization of the TrRPT2 Subunit

X. Chen1, A.J. Wood1
1 Department of Plant Biology, Southern Illinois University - Carbondale, Carbondale, USA

Tortula ruralis is an important experimental system for the study of plant desiccation tolerance. EST gene discovery efforts utilizing desiccated gametophytes have identified a cDNA TrRpt2 encoding a predicted polypeptide with significant similarity to the 26S proteasome regulatory subunit IV. TrRPT2, the 446 amino acid deduced polypeptide, has a predicted molecular mass of 49.6 kDa, and a predicted pI of 8.15. Phylogenetic analysis demonstrated that previously characterized RPT2 polypeptide sequences could be reproducibly grouped into 3 major clades and that TrRPT2 forms a discrete evolutionary group. RNA blot hybridizations were used to analyze TrRpt2 expression in response to: 1) desiccation and rehydration, 2) abscisic acid-treatment, 3) increased NaCl concentration, and 4) NaCl-shock. TrRpt2 steady-state mRNA transcript levels are unchanged in response to all treatments and the gene is constitutively expressed.

Keywords: ABA; bryophytes; desiccation; moss; salinity
Subjects: abscisic acid (ABA); bryophytes, resurrection plants; desiccation tolerance, TrRpt2 expression; mosses, resurrection plants; 26S proteasome; rehydration, TrRpt2 expression; resurrection plants; salt stress, resurrection plants; salt stress, TrRpt2 expression; Tortula muralis

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

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Chen, X., & Wood, A.J. (2003). The 26S Proteasome of the Resurrection Plant Tortula ruralis: Cloning and Characterization of the TrRPT2 Subunit. Biologia plantarum, 46(3), 363-368. doi: 10.1023/A:1024369917715
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