biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:367-371, 2003 | DOI: 10.1023/B:BIOP.0000023879.74558.48

Stress-Induced Changes in Peptidyl-Prolyl cis-trans Isomerase Activity of Sorghum bicolor Seedlings

A.D. Sharma1, N. Wajapeyee1, V. Yadav1, P. Singh1,*
1 Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, India

Developmental changes and effects of various abiotic stresses on peptidyl prolyl cis-trans isomerase (PPIase) activity were studied in the seedlings of sorghum [Sorghum bicolor (L.) Moench cv. CSH-6]. The PPIase activity of sorghum seedlings markedly decreased after two days of germination. Up to 90 % of the PPIase activity was inhibited by cyclosporin-A. Maximal increase in specific PPIase activity in the 3-d-old seedlings was observed in response to osmotic stress and it was transient in nature. The stress-induced enhancement in PPIase activity, depending upon tissue and stress treatment, was due to induction of cyclophilins as well as other PPIases. Osmotic stress-induced enhancement in PPIase activity in the drought susceptible cv. SPRU-94008B was maximal in roots, as compared to shoots in the drought tolerant cv. ICSV-272.

Keywords: abiotic stresses; cyclophilin; sorghum
Subjects: cultivar and genotype differences, abiotic stresses; cyclophyllin; peptidyl prolyl cis-trans isomerase; Sorghum bicolor

Published: November 1, 2003  Show citation

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Sharma, A.D., Wajapeyee, N., Yadav, V., & Singh, P. (2003). Stress-Induced Changes in Peptidyl-Prolyl cis-trans Isomerase Activity of Sorghum bicolor Seedlings. Biologia plantarum, 46(3), 367-371. doi: 10.1023/B:BIOP.0000023879.74558.48
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