biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:375-381, 2003 | DOI: 10.1023/A:1024326102694

Valerophenone synthase-like chalcone synthase homologues in Humulus lupulus

P. Novák1,2, J. Matoušek1, J. Bříza1
1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
2 Biological Faculty, South Bohemian University, České Budějovice, Czech Republic

Valerophenone synthase homologue of chalcone synthase (CHS) is the first key enzyme described to be involved in the biosynthesis of bitter acids, the compounds produced in hop lupulin glands valuable for the taste of beer. The complete sequence of a novel homologue of CHS chs 4 was isolated from hop. Protein predicted from chs 4 cDNA has 43.45 kDa and length 395 amino acids. It was found by the analysis of chs 4 flanking sequences that this gene is in the cluster with other CHS homologues - chs 3 and vps. The intron identified in chs 4 has been found to be homological to vps and chs 3 introns. Expression of chs 4 was partially characterized using reverse transcription polymerase chain reaction and it was found that chs 4 is specifically expressed in the glandular tissue of hop cones likewise vps. The predicted protein sequence CHS 4 was compared with other CHS-like proteins.

Keywords: bitter acids; flavonoids; hop; prenylated flavonoids
Subjects: bitter acids, hop; chalcone synthase; flavonoids; hop, bitter acids; hop, chalcone synthase; Humulus lupulus; prenylated flavonoids; valerophenone synthase-like chalcone synthase

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

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Novák, P., Matoušek, J., & Bříza, J. (2003). Valerophenone synthase-like chalcone synthase homologues in Humulus lupulus. Biologia plantarum, 46(3), 375-381. doi: 10.1023/A:1024326102694
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