biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:399-403, 2003 | DOI: 10.1023/A:1024386421350

Diurnal Variations in the Activity of Phosphoenolpyruvate Carboxylase and NADP-Malic Enzyme During the Early Steps of Interaction between Glycine max and Bradyrhizobium japonicum

M. Geneva1, Y. Markovska2, V. Vassileva1, G. Ignatov1
1 Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Department of Plant Physiology, Faculty of Biology, University of Sofia, Sofia, Bulgaria

Two important enzyme in organic acid metabolism, phosphoenolpyruvate carboxylase (PEPC) and NADP-malic enzyme (NADP-ME), show marked diurnal rhythms in their activities during the establishment of the soybean - B. japonicum symbiosis. The pH of the nutrient solution changes in parallel with NADP-ME activity, being maximal during the night periods, whereas activity of PEPC was highest during the day periods. The results from the experiments with stem girdled plants indicated that the activity of root PEPC is modulated to a great extent by the supply of photosynthates from the shoots. It was also established that succinate application in the nutrient solution during inoculation altered significantly the pattern of assayed enzyme activities. Although our experiments did not reveal the precise mechanism of the involvement of root PEPC and NADP-ME in soybean response to inoculation with B. japonicum, they indicated the pattern of their activity during the first 72 h postinoculation which are critical for establishment and functioning of the symbiosis.

Keywords: inoculation; medium pH; soybean roots; stem girdling; succinate
Subjects: bacteria; Bradyrhizobium japonicum; diurnal variations, photosynthetic enzymes; Glycine max; NADP-malic enzyme; phosphoenolpyruvate carboxylase; root, soybean-Bradyrhizobium symbiosis; soybean-Bradyrhizobium symbiosis; succinate, soybean-Bradyrhizobium symbiosis

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

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Geneva, M., Markovska, Y., Vassileva, V., & Ignatov, G. (2003). Diurnal Variations in the Activity of Phosphoenolpyruvate Carboxylase and NADP-Malic Enzyme During the Early Steps of Interaction between Glycine max and Bradyrhizobium japonicum. Biologia plantarum, 46(3), 399-403. doi: 10.1023/A:1024386421350
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