biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:421-424, 2003 | DOI: 10.1023/B:BIOP.0000023886.93651.b4

Esterase as a Marker to Study the Genetic Fidelity of Micropropagated Banana

Y. Ramalakshmi Dutta1, G. Gangopadhyay2, S. Das2, B.K. Dutta1, K.K. Mukherjee2,*
1 Sri Ramkrishna Ashram, Vivekananda Institute of Biotechnology, West Bengal, India
2 Cytogenetics Laboratory, Department of Botany, Bose Institute, West Bengal, India

Isozymic profiles of different micropropagated banana (Musa spp.) cultivars (Giant Governor, Dwarf Cavendish, Robusta, Champa, Kachakel and Chatim) of West Bengal, India were assessed at different subcultural passages. Variation with respect to the banding pattern was noticed only in esterase but not in peroxidase and acid phosphatase. Of the six cultivars, four showed variation both at isozymic and yield level. Two cultivars (Kachakel and Chatim) maintained their esterase profile and genetic stability even after twenty subcultural passages.

Keywords: acid phosphatase; isozyme; Musa spp; peroxidase; subcultural passage
Subjects: banana, esterase, micropropagation; cultivar and genotype differences, isozymic profiles of micropropagated banana; esterases; in vitro culture, micropropagation; markers, genetic; Musa acuminata; Musa balbisiana; phosphatases

Published: November 1, 2003  Show citation

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Ramalakshmi Dutta, Y., Gangopadhyay, G., Das, S., Dutta, B.K., & Mukherjee, K.K. (2003). Esterase as a Marker to Study the Genetic Fidelity of Micropropagated Banana. Biologia plantarum, 46(3), 421-424. doi: 10.1023/B:BIOP.0000023886.93651.b4
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