biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:423-427, 2003 | DOI: 10.1023/A:1024316107237

Growth and Nitrate Reductase Activity of Chlorella fusca Cells as Affected by Long Term Salinity

A.A. Shafea1
1 Botany Department, Faculty of Science, Assiut University, Assiut, Egypt

Influence of various saline media on Chlorella fusca growth, contents of photosynthetic pigments, and activity of the nitrate reductase (NRA) was determined. KCl, CaCl2, and MgCl2 in concentrations relative to NaCl as 1:1, 2:1, and 3:1 promote cell number, dry mass, and contents of photosynthetic pigments. The activity of NR was enhanced by Mg2+ and K+ and in some cases by Ca2+ at all ratios applied.

Keywords: calcium chloride; magnesium chloride; potassium chloride; sodium chloride
Subjects: algae; calcium chloride, long-term salinity, nitrate reductase; carotenoids, salt stress; Chlorella fusca; chlorophyll, salt stress; magnesium chloride, long-term salinity, nitrate reductase; nitrate reductase; potassium chloride, long-term salinity, nitrate reductase; salt stress, growth

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

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Shafea, A.A. (2003). Growth and Nitrate Reductase Activity of Chlorella fusca Cells as Affected by Long Term Salinity. Biologia plantarum, 46(3), 423-427. doi: 10.1023/A:1024316107237
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    References

    1. Abdel-Basset, R.: Photosynthesis and some related metabolic processes as influenced by salinization treatments.-Ph.D. Thesis. Faculty of Science, Assiut University, Assiut 1986.
    2. Abdel-Fatah, Z.: Interactive effects of salinity and nitrogen source on photosynthesis and some related metabolic processes of Scenedesmus obliquus.-Ph.D. Thesis. Faculty of Science, Assiut University, Assiut 1988.
    3. Ahmed, A.M., Mohammed, A.A., Heikal, M.D., Mohammed, R.H.: Physiology of some Nile algae. 1. Effect of increased NaCl concentration in the medium.-Acta hydrobiol. 27: 25-32, 1985.
    4. Anil, R., Kavita, S., Dubey, R.S.: Nitrate reductase from rice seedlings: Partial purification, characterization and the effects of in situ and in vitro NaCl salinity.-J. Plant Physiol. 151: 316-322, 1997. Go to original source...
    5. Bange, G.G.: A comparison of the effect of Ca and Mg on the separate components of alkali cation uptake in excised barley roots.-Plant Soil 18: 177-181, 1968. Go to original source...
    6. Bardzik, J.M., Marsh, H.V., Jr., Havis, J.R.: Effects of water stress on the activities of three enzymes in maize seedlings.-Plant Physiol. 47: 828-831, 1971. Go to original source...
    7. Bazhanova, N.V., Maslova, T.G., Popova, I.N., Sapozhnikov, D.I., Eidelman, Z.M.: [Plastid pigments of green plants and methods of investigating them.]-Nauka, Moskva 1964. [In Russ.]
    8. Beevers, L., Hageman, R.H.: Nitrate reduction in higher plants.-Plant Physiol. 20: 495-522, 1969. Go to original source...
    9. Bernstein, L.: Effects of salinity and sodicity on plant growth.-Annu. Rev. Phytopathol. 13: 296-312, 1975. Go to original source...
    10. Cramer, G.R., Epstein, E., Läuchli, A.: Kinetics of root elongation of maize in response to short-term exposure to NaCl and elevated calcium concentration.-J. exp. Bot. 39: 1513-1522, 1988. Go to original source...
    11. Czernas, K.: The use of selected alga tests in determining the influence of potassium and sodium on the eutrophication of waters.-Acta hydrobiol. 20: 323-344, 1978.
    12. Flowers, T.J., Hall, J.L. Ward, M.E.: Salt tolerance in the halophyte saueda maritima. Further properties of the enzyme malate dehydrogenates.-Phytochemistry 15: 1231-1234, 1976. Go to original source...
    13. Gale, J.: Water balance and gas exchange of plants under saline conditions.-In: Poljakoff-Mayber, A., Gale, J. (ed.): Plants in Saline Environment. Pp. 168-185. Springer-Verlag, Berlin-Heidelberg-New York 1975. Go to original source...
    14. García-Gonzáles, M., Sánchez-Maeso, E., Quesada, A., Fernández-Valiente, E.: Sodium requirement for photosynthesis and nitrate assimilation in a mutant of Nostoc muscorum.-J. Plant Physiol. 127: 423-429, 1987. Go to original source...
    15. Gary-Bobo, C.M.: Effect of Ca2+ on the water and non-electrolyte permeability of phospholipid membranes.-Nature 228: 1101-1102, 1970. Go to original source...
    16. Gimmler, H.; Schirling, R.: Cation permeability of plasma lemma of the halotolerant alga Dunaliella parva II. Cation content and glycerol concentration of the cells as dependent upon extra cellular NaCl concentration.-Z. Pflanzenphysiol. 87: 435-444, 1978. Go to original source...
    17. Greenway, H., Munns, R.: Mechanism of salt tolerance in non-halophytes.-Annu. Rev. Plant Physiol. 31: 149-190, 1980. Go to original source...
    18. Hewitt, E.J.: Assimilatory nitrate-nitrite reduction.-Annu. Rev. Plant Physiol. 26: 73-100, 1975. Go to original source...
    19. Jaworski, E.G.: Nitrate reductase assay in intact plant tissues.-Biochem. biophys. Res. Commun. 43: 377-379, 1971. Go to original source...
    20. Kaiser, W.M., Weber, H., Sauer, M.: Photosynthetic capacity, osmotic response and solute content of leaves and chloroplasts from Spinacia oleracea under salt stress.-Z. Pflanzenphysiol. 113: 15-27, 1983. Go to original source...
    21. Kingsbury, R.W., Epstein, E.: Salt sensitivity in wheat. A case for specific ion toxicity.-Plant Physiol 80: 651-654, 1986. Go to original source...
    22. Kinraide, T.B.: Interactions among Ca2+, Na+ and K+ in salinity toxicity. quantitative resolution of multiple toxic and ameliorative effects.-J. exp. Bot. 50: 1495-1505, 1999. Go to original source...
    23. Kleinkopf, P.A., Wallace, A.: Physiological basis for salt tolerance in Tamarix ramossissima.-Plant Sci. Lett. 3: 157-163, 1974. Go to original source...
    24. Leopold, A.C., Willing, R.P.: Evidence for toxicity effects of salt on membranes.-In: Staples, R.C., Toennissen, G.H. (ed.): Salinity Tolerance in Plant. Strategies for Crop Improvement. Wiley Interscience Publ., New York-Chichester-Brisbane-Toronto-Singapore 1984.
    25. Metzner, H., Rau, H., Senger, H.: Untersuchungen zur Synchronisierbarkeit einzelner Pigmentmangel Mutanten von Chlorella.-Planta 65: 186-194, 1965. Go to original source...
    26. Mohammed, A.A., Shafea, A.A.: Growth and some metabolic activities of Scenedesmus obliquus cultivated under different NaCl concentrations.-Biol. Plant. 34: 423-430, 1992. Go to original source...
    27. Munns, R., Termaat, A.: Whole plant responses to salinity.-Aust. J. Plant Physiol. 13: 143-160, 1986. Go to original source...
    28. Passera, C., Albuzio, A.: Effect of salinity on photosynthesis and photorespiration on two wheat species (Triticum durum cv. PEPE 2122 and Triticum aestivum cv. Marzotto).-Can. J. Bot. 56: 121-126, 1978. Go to original source...
    29. Pate, J.S.: Transport and portioning of nitrogenous solutes.-Annu. Rev. Plant Physiol. 31: 313-340, 1980. Go to original source...
    30. Peuke, D.A., Jeschke, D.W.: The characterization of inhibition of net nitrate uptake by salt in salt-tolerant barley (Hordeum vulgare L. cv. California Mariout).-J. exp. Bot. 50: 1365-1372, 1999. Go to original source...
    31. Plaut, Z.: Nitrate reductase activity of wheat seedlings during exposure to and recovery from water stress and salinity.-Physiol. Plant. 30: 212-217, 1974. Go to original source...
    32. Schubert, H., Fluda, S. Hagemann, M.: Effects of adaptation to different salt concentrations on photosynthesis and pigmentation of the cyanobacterium Synechocystis sp. PCC 6803.-J. Plant Physiol. 142: 291-295, 1993. Go to original source...
    33. Seemann, J.R., Critchley, C.: Effects of salt stress on the growth, ion content, stomatal behaviours and photosynthetic capacity of a salt sensitive species Phaseolus vulgaris L.-Planta 164: 151-162, 1985. Go to original source...
    34. Setter, T.L.: Response of Chlorella to High NaCl and Neutral Osmotica.-M.Sc. Thesis. University of Western Australia, Adelaide 1979.
    35. Setter, T.L., Greenway, H.: Growth and osmoregulation of Chlorella emersonii in NaCl and neutral osmotica.-Aust. J. Plant Physiol. 6: 47-60, 1979. Go to original source...
    36. Shafea, A.A.: Physiological Response of Algal Cells to Sodium Chloride Salinization with a Special Reference to Proline Accumulation.-M.Sc. Thesis. Bot. Dept., Fac. Sci., Assiut University, Assiut 1987.
    37. Shieh, Y.J., Barber, J.: Intracellular sodium and potassium concentrations and net cation movements in Chlorella pyrenoidosa.-Biochim. biophys. Acta 233: 594-603, 1971. Go to original source...
    38. Sinha, S.K., Nicholas, J.D.: Nitrate reductase.-In: Paleg, L.G., Aspinall, D. (ed): The Physiology and Biochemistry of Drought Resistance in Plants. Pp. 145-168. Academic Press, Sydney 1981.
    39. Stein, J.R.: Growth and mating of Gonium pectorale (Volvocales) in defined media.-J. Phycol. 2: 23-28, 1966. Go to original source...
    40. Strogonov, B.P.: Fiziologicheskie Osnovy Soleustoïchivosti Rasteniï. [Physiological Bases of Salt Tolerance in Plants.]-Izd. Akad. Nauk SSSR, Moskva 1962. [In Russ.]
    41. Sweby, D.L., Huchkett, B.I., Watt, M.P.: Effect of nitrogen nutrition on salt-stessed Nicotiana tabacumvar. Samsun in vitro plantlets.-J. exp. Bot. 276: 995-1008, 1994. Go to original source...
    42. Weimberg, R.: Modification of foliar solute concentrations of calcium in two species of wheat stressed with sodium chloride and/or potassium chloride.-Physiol. Plant. 73: 418-425, 1988. Go to original source...

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