biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:425-427, 2003 | DOI: 10.1023/B:BIOP.0000023887.16716.f7

Effects of Antibiotics and Bialaphos on the Growth and Development of Embryogenic Callus Cultures of Muscari armeniacum

S. Suzuki1, M. Nakano1,*
1 Faculty of Agriculture, Niigata University, Niigata, Japan

Effects of 4 potentially selective agents for transformed cells, 3 antibiotics [kanamycin, geneticin (G418) and hygromycin] and bialaphos, as well as 2 antibiotics for eliminating Agrobacterium, carbenicillin and cefotaxime on growth and somatic embryogenesis of embryogenic calli of Muscari armeniacum cv. Blue Pearl were evaluated. Callus growth was completely inhibited by 75 mg dm-3 hygromycin or 4 mg dm-3 bialaphos, and somatic embryos were never produced on media containing 25 mg dm-3 hygromycin or 3 mg dm-3 bialaphos. Kanamycin and G418 less inhibited growth and somatic embryogenesis of the calli. On the contrary, carbenicillin and cefotaxime promoted both callus growth and somatic embryogenesis at all concentrations tested.

Keywords: genetic transformation; grape hyacinth; selective agent; somatic embryogenesis
Subjects: antibiotics, callus culture; bialaphos; embryo culture, antibiotics; grape hyacinth, genetic transformation; in vitro culture, embryonic callus culture; in vitro culture, nutrient media modification; in vitro culture, somatic embryogenegis; Muscari armeniacum

Published: November 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Suzuki, S., & Nakano, M. (2003). Effects of Antibiotics and Bialaphos on the Growth and Development of Embryogenic Callus Cultures of Muscari armeniacum. Biologia plantarum, 46(3), 425-427. doi: 10.1023/B:BIOP.0000023887.16716.f7
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bhau, B.S., Wakhlu, A.K.: Effect of some antibiotics on the in vitro morphogenetic response from callus cultures of Coryphanta elephantidens.-Biol. Plant. 44: 19-24, 2001. Go to original source...
    2. Dekeyser, R., Claes, B., Marichal, M., Van Montagu, M., Caplan, A.: Evaluation of selectable makers for rice transformation.-Plant Physiol. 90: 217-223, 1989. Go to original source...
    3. Dennehey, B.K., Petersen, W.L., Ford-Santino, C., Pajeau, M., Armstrong, C.L.: Comparison of selective agents for use with the selectable marker gene bar in maize transformation.-Plant Cell Tissue Organ. Cult. 36: 1-7, 1994. Go to original source...
    4. Eady, C.C., Lister, C.E.: A comparison of four selective agents for use with Allium cepa L. immature embryos and immature embryo-derived cultures.-Plant Cell Rep. 18: 117-121, 1998. Go to original source...
    5. Holford, P., Newbury, H.J.: The effect of antibiotics and their breakdown products on the in vitro growth of Antirrhinum majus.-Plant Cell Rep. 11: 93-96, 1992. Go to original source...
    6. Mathias, R.J., Boyd, L.A.: Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticum aestivum L. EM. Thell).-Plant Sci. 46: 217-223, 1986. Go to original source...
    7. Nakano, M., Mii, M.: Antibiotics stimulate somatic embryogenesis without plant growth regulators in several Dianthus cultivars.-J. Plant Physiol. 141: 721-725, 1993. Go to original source...
    8. Pius, J., George, L., Eapen, S., Rao, P.S.: Enhanced plant regeneration in pearl millet (Pennisetum americanum) by ethylene inhibitors and cefotaxime.-Plant Cell Tissue Organ Cult. 32: 91-96, 1993. Go to original source...
    9. Pollock, K., Barfield, D.G., Shields, R.: The toxicity of antibiotics to plant cell cultures.-Plant Cell Rep. 2: 36-39, 1983. Go to original source...
    10. Potrykus, I., Saul, M.W., Petruska, J., Paszkowski, J., Shillito, R.D.: Direct gene transfer to cells of a graminaceous monocot.-Mol. gen. Genet. 199: 183-188, 1985. Go to original source...
    11. Suzuki, S., Nakano, M.: Organogenesis and somatic embryogenesis from callus cultures in Muscari armeniacum Leichtl. ex Bak.-In Vitro cell. dev. Biol. 37: 382-387, 2001. Go to original source...
    12. Suzuki, S., Niimi, Y., Sakakibara, T., Hosokawa, K., Yamamura, S., Nakano, M.: Effects of several antibiotics and bialaphos on the growth and organ formation of Lilium formosanum calli and transient expression of the gusA gene after co-cultivation with Agrobacterium tumefaciens.-Plant Biotechnol. 15: 213-216, 1998. Go to original source...
    13. Suzuki, S., Oota, M., Nakano, M.: Embryogenic callus induction from leaf explants of the Liliaceous ornamental plant, Agapanthus praecox ssp. orientalis (Leighton) Leighton: Histological study and response to selective agents.-Sci. Hort. 95: 123-132, 2002. Go to original source...
    14. Wilmink, A., Dons, J.J.M.: Selective agents and marker genes for use in transformation of monocotyledonous plants.-Plant mol. Biol. Rep. 11: 165-185, 1993. Go to original source...

    Return to the content