biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:435-443, 2003 | DOI: 10.1023/A:1024350808146

Fractionation and Electrophoretic Patterns of Storage Proteins of Ebenus cretica. A preliminary Survey as a Tool in Taxonomy

T. Syros1, T. Yupsanis2, A. Economou1
1 School of Agriculture, Department of Horticulture, Aristotle University, Thessaloniki, Greece
2 School of Chemistry, Department of Biochemistry, Aristotle University, Thessaloniki, Greece

Seed storage proteins of Ebenus cretica were fractionated to albumins, globulins, prolamins and glutelins according to their solubility in water, 0.5 M NaCl solution, 55 % propanol-2 and 0.125 M sodium borate (pH 9.0) containing 0.5 % SDS (sodium dodecyl sulfate) solution, respectively. Glutelins consist of the major (about 81 %) fraction of the total extracted proteins. Analysis by SDS-PAGE revealed that the total extracted protein patterns from different racemes of the same plant were similar, while those from seeds of different plants were different. In addition, distinct differences were observed within protein patterns of alkaline extractable glutelin fractions and salt soluble globulin fractions. In E. cretica four ecotypes (A - D) were distinguished by SDS-PAGE of total extracted seed proteins. The last method was more simple and rapid than others and was suggested for screening analysis.

Keywords: albumins; globulins; glutelins; Leguminosae; ornamental plant; prolamins; SDS-PAGE
Subjects: albumins, storage proteins, seed; Ebenus cretica; globulins, storage proteins, seed; glutelins, storage proteins, seed; Leguminosae; prolamins, storage proteins, seed; storage proteins, tool in taxonomy

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

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Syros, T., Yupsanis, T., & Economou, A. (2003). Fractionation and Electrophoretic Patterns of Storage Proteins of Ebenus cretica. A preliminary Survey as a Tool in Taxonomy. Biologia plantarum, 46(3), 435-443. doi: 10.1023/A:1024350808146
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