biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:441-444, 2003 | DOI: 10.1023/B:BIOP.0000023891.16785.fc

Mannose: A Potential Selection System for Genetic Transformation of Annatto

V.B. Paiva Neto1, C.R. Carvalho2, W.C. Otoni1
1 Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brasil
2 Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brasil

The aim of the present work was to evaluate the feasibility of mannose as a selection system for the future genetic transformation of annatto (Bixa orellana L.). Hypocotyl segments, inverted hypocotyls and immature zygotic embryos were inoculated onto a Murashige and Skoog's medium supplemented with B5 vitamins, 87.6 mM sucrose and mannose in different combinations, 2.8 g dm-3 Phytagel®, and 4.56 μM zeatin (organogenesis) or 2.26 μM 2,4-dichlorophenoxyacetic acid and 4.52 μM kinetin (somatic embryogenesis). Annatto explants did not regenerate on medium with mannose as the only carbon source when inverted hypocotyls and immature zygotic embryos were used. However, organogenesis (5 % frequency) occurred exclusively in hypocotyl-derived explants nearest to the crown (collar) of the seedlings. No further shoot development was achieved. Therefore the substitution of sucrose by mannose inhibited both organogenesis and embryogenesis, and thus the employment of mannose could constitute an alternative selective agent in protocols for genetic transformation of this species.

Keywords: Bixa orellana; organogenesis; phosphomannose isomerase; somatic embryogenesis
Subjects: annatto, genetic transformation; Bixa orellana; genetic transformation; immature embryo culture, plant regeneration; in vitro culture, somatic embryogenegis; mannitol, mannose, source in in vitro culture; markers, genetic; phosphomannose isomerase; saccharide genetic transformation, mannose; transgenic plants

Published: November 1, 2003  Show citation

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Paiva Neto, V.B., Carvalho, C.R., & Otoni, W.C. (2003). Mannose: A Potential Selection System for Genetic Transformation of Annatto. Biologia plantarum, 46(3), 441-444. doi: 10.1023/B:BIOP.0000023891.16785.fc
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