biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:451-453, 2003 | DOI: 10.1023/A:1024354909055

Differential Expression of Peroxidase Isoenzymes in Soybean Roots Treated with the Benzothiadiazole

L.V. Hoffmann1, M.T.V. Carvalho2, E.J.B. Nogueira Cardoso3
1 Embrapa Algodão, Campina Grande, Brazil
2 Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, Brazil
3 Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil

The protection compound benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) was applied to soybean roots or leaves in a dose of 20 cm3 of a solution containing 25 μg g-1 of the active ingredient. Electrophoretic profiles of chitinase and superoxide dismutase were not altered by the product. Increased activity of two root anionic peroxidases and three differential isoforms of these enzymes were observed in plants with roots treated by BTH, which can be used as biochemical markers of the BTH effect.

Keywords: Glycine max; systemic acquired resistance
Subjects: benzothiadiazole; chitinase; peroxidase; superoxide dismutase; systemic acquired resistance

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Hoffmann, L.V., Carvalho, M.T.V., & Nogueira Cardoso, E.J.B. (2003). Differential Expression of Peroxidase Isoenzymes in Soybean Roots Treated with the Benzothiadiazole. Biologia plantarum, 46(3), 451-453. doi: 10.1023/A:1024354909055
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-54, 1976. Go to original source...
    2. Brune, W., Alfenas, A.C. (ed.): Eletroforese de isoenzimas e proteinas afins, fundamentos e aplicações em plantas e microrganismos. [Electrophoresis of isoenzymes and isoenzyme-like proteins, fundamentals and applications in plants and microorganisms].-Editora Universidade Federal de Viçosa, Viçosa 1998. [In Port.]
    3. Davis, B.J.: Disc electrophoresis II. Method and applications to human serum proteins.-Annu. Rev. N.Y. Acad. Sci. 121: 407-27, 1964. Go to original source...
    4. Friedrich, L., Lawton, K., Ruess, W., Masner, P., Specker, N., Rella, M.G., Meier, B., Dincher, S., Staub, T., Uknes, S., Métraux, J., Kessmann, H., Ryals, J.: A benzothiadiazole derivate induces systemic acquired resistance in tobacco.-Plant J. 10: 61-70, 1996. Go to original source...
    5. Görlach, J., Volrath, S., Knauf-Beiter, G., Hengy, G., Beckhove, U., Kogel, K., Oostendorp, M., Staub, T., Ward, E., Kessmann, H., Ryals, J.: Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.-Plant Cell 8: 629-643, 1996. Go to original source...
    6. Hammond-Kosack, K.E., Jones, J.D.G.: Resistance gene-dependent plant defense responses.-Plant Cell 8: 1773-1791, 1996. Go to original source...
    7. Lawton, K.A., Friedrich, L., Hunt, M., Weymann, K., Delaney, T., Kessmann, H., Staub, T., Ryals, J.: Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transdution pathway.-Plant J. 10: 71-82, 1996. Go to original source...
    8. Macko, V., Honold, G.R., Stahmann, M.A.: Soluble proteins and multiple enzyme forms in early growth of wheat.-Phytochemistry 6: 465-471, 1967. Go to original source...
    9. Rudolph, K., Stahmann, M.A.: Interactions of peroxidases and catalases between Phaseolus vulgaris and Pseudomonas phaseolicola (halo blight of bean).-Nature 204: 474-475, 1964. Go to original source...
    10. Siegrist, J., Glenewinkel, D., Kolle, C., Schmidtke, M.: Chemically induced resistance in green bean against bacterial and fungal pathogens.-J. Plant Dis. Protect. 104: 599-610, 1997.
    11. Trudel, J., Asselin, A.: Detection of chitinase activity after polyacrylamide gel eletrophoresis.-Anal. Biochem. 175: 362-366, 1989. Go to original source...
    12. Vance, C.P., Kirk, T.K., Sherwood, R.T.: Lignification as a mechanism of disease resistance.-Annu. Rev. Phytopathol. 18: 259-288, 1980. Go to original source...
    13. Van Loon, L.C.: Tobacco polyphenoloxidases: A specific staining method indicating non identity with peroxidases.-Phytochemistry 10: 503-507, 1971. Go to original source...

    Return to the content