biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:459-461, 2003 | DOI: 10.1023/A:1024359126802

Micropropagation of Tectona grandis: Assessment of Genetic Fidelity

G. Gangopadhyay1, S. Basu Gangopadhyay2, R. Poddar1, S. Gupta1, K.K. Mukherjee1
1 Cytogenetics Laboratory, Department of Botany, Bose Institute, West Bengal, India
2 Department of Botany, Brahmananda Keshab Chandra College, West Bengal, India

Random amplified polymorphic DNA (RAPD) markers were used to analyze genetic fidelity of micropropagated teak (Tectona grandis L.) clones with respect to subcultural passage. Of the twenty primers screened, no variation in RAPD profiles was noticed in the in vitro clones of fifth, tenth, fifteenth and twentieth passage in comparison to the in vivo mother plants. Only one micropropagated plant of twenty-fifth subcultural passage, however, differed from the in vivo ones. It revealed the appearance of a new polymorphic DNA fragment (molecular mass 379 kb) in case of primer OPB-08. This primer, manifesting detectable variation, may be utilized as a diagnostic marker for assessing genetic fidelity of micropropagted teak plants.

Keywords: random amplified polymorphic DNA; teak
Subjects: in vitro culture, micropropagation; random amplified polymorphic DNA (RAPD); teak, micropropagation; Tectona grandis

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Gangopadhyay, G., Basu Gangopadhyay, S., Poddar, R., Gupta, S., & Mukherjee, K.K. (2003). Micropropagation of Tectona grandis: Assessment of Genetic Fidelity. Biologia plantarum, 46(3), 459-461. doi: 10.1023/A:1024359126802
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Chaterjee, G., Prakash, J.: Genetic stability in commercial tissue culture.-In: Prakash, J., Pierik, R.L.M. (ed.): Plant Biotechnology: Commercial Prospects and Problems. Pp. 111-121. Oxford IBH Publishing Co., New Delhi-Bombay-Calcutta 1996.
    2. De Klerk, G.J.: How to measure somaclonal variation.-Acta bot. 39: 129-144, 1990. Go to original source...
    3. Devi, Y.S., Gupta, S., Mukherjee, B.B.: Effect of sucrose on growth and chlorophyll synthesis in mixotrophic culture.-J. Plant Biochem. Biotechnol. 7: 57-59, 1998. Go to original source...
    4. Devi, Y.S., Mukherjee, B.B., Gupta, S.: Rapid cloning of elite teak (Tectona grandis Linn.) by in vitro multiple shoot production.-Indian J. exp. Biol. 32: 668-671, 1994.
    5. Ford-Lloyd, B.: Measuring genetic variation using molecular markers.-(http://www.cgiar.org/ipgri), 1996.
    6. Gangopadhyay, G., Das, S., Mitra, S.K., Poddar, R., Modak, B.K., Mukherjee, K.K.: Enhanced rate of multiplication and rooting through the use of coir in aseptic liquid culture media.-Plant Cell Tissue Organ Cult. 68: 301-310, 2002. Go to original source...
    7. Goto, S., Thakur, R.C., Ishii, K.: Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers.-Plant Cell Rep. 18: 193-197, 1998. Go to original source...
    8. Gupta, P.K., Varshney, R.K.: Molecular markers for genetic fidelity during micropropagation and germplasm conservation?-Curr. Sci. 76: 1308-1310, 1999.
    9. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    10. Rahman, M.H., Rajora, O.P.: Microsatellite DNA somaclonal variation in micropropagated trembling aspen (Populus tremuloides).-(http://link.springer.de/link/service/journals/00299/contents/01/00365/s002990100365ch002.html), 2001.
    11. Ramalakshmi Dutta, Y., Gangopadhyay, G., Das, S., Dutta, B.K., Mukherjee, K.K.: Esterase as a marker to study the genetic fidelity of micropropagated banana.-Biol. Plant. 2003 (in press). Go to original source...
    12. Rani, V., Parida, A., Raina, S.N.: Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh.-Plant Cell Rep. 14: 459-462, 1995. Go to original source...
    13. Rogers, S.O., Bendich, A.J.: Extraction of DNA from plant tissues.-In: Gelvin, S.P., Schilperoort, R.A. (ed.): Plant Molecular Biology Manual. Pp. A6:1. Kluwer Academic Publishers, Dordrecht 1988. Go to original source...
    14. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V.: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.-Nucl. Acids Res. 18: 6531-6535, 1990. Go to original source...

    Return to the content