biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:463-465, 2003 | DOI: 10.1023/A:1024363210872

In vitro Shoot Regeneration from Flower and Leaf Explants in Rhododendron

S. Tomsone1, D. Gertnere1
1 Plant Tissue Culture Laboratory, University of Latvia, Latvia

Rhododendron shoot regeneration was accomplished using either flower explants (each consisting of ovary with pedicel) of Rhododendron cvs. Nova Zembla and Irina or leaves isolated from in vitro grown Rhododendron catawbiense Michx. Multiple shoot tip clumps were obtained on Anderson's medium containing 0.5 to 1.5 mg dm-3 thidiazuron (TDZ) in combination with 12 to 15 mg dm-3 N6-[2-isopentenyl]adenine (2iP) and 1 to 3 mg dm-3 indole-3-butyric acid (IBA). After 16 weeks on the regeneration media, explants with shoot tip clumps were transferred for shoot elongation to Anderson's medium with 3 mg dm-3 2iP. Two months later, the shoots have reached 5 to 40 mm in length and were fit for subcultivation.

Keywords: indole-3-butyric acid; micropropagation; N6-[2-isopentenyl]adenine; thidiazuron
Subjects: flower, explants; in vitro culture, nutrient media modification; in vitro culture, plant regeneration, organogenesis; in vitro culture, regeneration from flower and leaf explants; Rhododendron satawbiense

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

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Tomsone, S., & Gertnere, D. (2003). In vitro Shoot Regeneration from Flower and Leaf Explants in Rhododendron. Biologia plantarum, 46(3), 463-465. doi: 10.1023/A:1024363210872
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